1887

Abstract

An aerobic, pink-pigmented, budding bacterium, designated strain S26, was isolated from an acidic peat bog of north-western Russia. Cells were non-motile and spherical, occurring singly, in pairs or in short chains, and were able to attach to surfaces by means of a holdfast material. Strain S26 was a moderately acidophilic, mesophilic organism capable of growth at pH 3.2–7.1 (optimum at pH 4.8–5.0) and at 4–33 °C (optimum at 20–26 °C). Most sugars, several organic acids and polyalcohols were the preferred growth substrates. The major fatty acids were C, Cω9 and Cω6,12. The major neutral lipids were n-C hydrocarbon and squalene; the polar lipids were phosphatidylglycerol, phosphatidylcholine and components with an unknown structure. The DNA G+C content of strain S26 was 62.2 mol%. 16S rRNA gene sequence analysis showed that strain S26 is a member of the order . Among taxonomically characterized representatives of this order, highest levels of 16S rRNA gene sequence similarity (95.1–95.2 %) were observed with strains of the non-filamentous, peat-inhabiting planctomycete . Strain S26 could be differentiated from based on pigmentation, significant differences in substrate utilization patterns, greater tolerance of acidic conditions and the presence of Cω9. Based on the data presented, strain S26 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed; the type strain is S26 ( = DSM 23044 = VKM B-2599).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.025924-0
2012-01-01
2020-01-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/62/1/118.html?itemId=/content/journal/ijsem/10.1099/ijs.0.025924-0&mimeType=html&fmt=ahah

References

  1. Bodelier P. L. E. , Gillisen M. J. , Hordijk K. , Damsté J. S. , Rijpstra W. I. C. , Geenevasen J. A. J. , Dunfield P. F. . ( 2009; ). A reanalysis of phospholipid fatty acids as ecological biomarkers for methanotrophic bacteria. . ISME J 3:, 606–617. [CrossRef] [PubMed]
    [Google Scholar]
  2. Bondoso J. , Albuquerque L. , Nobre M. F. , Lobo-da-Cunha A. , da Costa M. S. , Lage O. M. . ( 2011; ). Aquisphaera giovannonii gen. nov., sp. nov., a novel planctomycete isolated from a freshwater aquarium. . Int J Syst Evol Microbiol 61:, 2844–2850. [CrossRef] [PubMed]
    [Google Scholar]
  3. Dedysh S. N. , Pankratov T. A. , Belova S. E. , Kulichevskaya I. S. , Liesack W. . ( 2006; ). Phylogenetic analysis and in situ identification of bacteria community composition in an acidic Sphagnum peat bog. . Appl Environ Microbiol 72:, 2110–2117. [CrossRef] [PubMed]
    [Google Scholar]
  4. Fuerst J. A. . ( 1995; ). The planctomycetes: emerging models for microbial ecology, evolution and cell biology. . Microbiology 141:, 1493–1506. [CrossRef] [PubMed]
    [Google Scholar]
  5. Fuerst J. A. . ( 2005; ). Intracellular compartmentation in planctomycetes. . Annu Rev Microbiol 59:, 299–328. [CrossRef] [PubMed]
    [Google Scholar]
  6. Gerhardt P. . ( 1981; ). Manual of Methods for General Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  7. Giovannoni S. J. , Schabtach E. , Castenholz R. W. . ( 1987; ). Isosphaera pallida, gen. nov., and comb. nov., a gliding, budding eubacterium from hot springs. . Arch Microbiol 147:, 276–284. [CrossRef]
    [Google Scholar]
  8. Kulichevskaya I. S. , Pankratov T. A. , Dedysh S. N. . ( 2006; ). Detection of representatives of the Planctomycetes in Sphagnum peat bogs by molecular and cultivation approaches. . Microbiology (English translation of Mikrobiologiya) 75:, 329–335. [CrossRef] [PubMed]
    [Google Scholar]
  9. Kulichevskaya I. S. , Ivanova A. O. , Belova S. E. , Baulina O. I. , Bodelier P. L. E. , Rijpstra W. I. C. , Sinninghe Damsté J. S. , Zavarzin G. A. , Dedysh S. N. . ( 2007; ). Schlesneria paludicola gen. nov., sp. nov., the first acidophilic member of the order Planctomycetales, from Sphagnum-dominated boreal wetlands. . Int J Syst Evol Microbiol 57:, 2680–2687. [CrossRef] [PubMed]
    [Google Scholar]
  10. Kulichevskaya I. S. , Ivanova A. O. , Baulina O. I. , Bodelier P. L. E. , Damsté J. S. , Dedysh S. N. . ( 2008; ). Singulisphaera acidiphila gen. nov., sp. nov., a non-filamentous, Isosphaera-like planctomycete from acidic northern wetlands. . Int J Syst Evol Microbiol 58:, 1186–1193. [CrossRef] [PubMed]
    [Google Scholar]
  11. Kulichevskaya I. S. , Baulina O. I. , Bodelier P. L. E. , Rijpstra W. I. C. , Damsté J. S. , Dedysh S. N. . ( 2009; ). Zavarzinella formosa gen. nov., sp. nov., a novel stalked, Gemmata-like planctomycete from a Siberian peat bog. . Int J Syst Evol Microbiol 59:, 357–364. [CrossRef] [PubMed]
    [Google Scholar]
  12. Ludwig W. , Strunk O. , Westram R. , Richter L. , Meier H. , Yadhukumar , Buchner A. , Lai T. , Steppi S. . & other authors ( 2004; ). arb: a software environment for sequence data. . Nucleic Acids Res 32:, 1363–1371. [CrossRef] [PubMed]
    [Google Scholar]
  13. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  14. Owen R. J. , Hill L. R. , Lapage S. P. . ( 1969; ). Determination of DNA base compositions from melting profiles in dilute buffers. . Biopolymers 7:, 503–516. [CrossRef] [PubMed]
    [Google Scholar]
  15. Schlesner H. . ( 1994; ). The development of media suitable for the microorganisms morphologically resembling Planctomyces spp., Pirellula spp., and other Planctomycetales from various aquatic habitats using dilute media. . Syst Appl Microbiol 17:, 135–145.[CrossRef]
    [Google Scholar]
  16. Sinninghe Damsté J. S. , Rijpstra W. I. C. , Schouten S. , Fuerst J. A. , Jetten M. S. M. , Strous M. . ( 2004; ). The occurrence of hopanoids in planctomycetes: implications for the sedimentary biomarker record. . Org Geochem 35:, 561–566. [CrossRef]
    [Google Scholar]
  17. Staley J. T. , Fuerst J. A. , Giovannoni S. , Schlesner H. . ( 1992; ). The order Planctomycetales and the genera Planctomyces, Pirellula, Gemmata and Isosphaera . . In The Prokaryotes: a Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, , 2nd edn., pp. 3710–3731. Edited by Balows A. , Truper H. , Dworkin M. , Harder W. , Schleifer K. H. . . New York:: Springer;.
    [Google Scholar]
  18. Sturt H. F. , Summons R. E. , Smith K. , Elvert M. , Hinrichs K.-U. . ( 2004; ). Intact polar membrane lipids in prokaryotes and sediments deciphered by high-performance liquid chromatography/electrospray ionization multistage mass spectrometry—new biomarkers for biogeochemistry and microbial ecology. . Rapid Commun Mass Spectrom 18:, 617–628. [CrossRef] [PubMed]
    [Google Scholar]
  19. Wang J. , Jenkins C. , Webb R. I. , Fuerst J. A. . ( 2002; ). Isolation of Gemmata-like and Isosphaera-like planctomycete bacteria from soil and freshwater. . Appl Environ Microbiol 68:, 417–422. [CrossRef] [PubMed]
    [Google Scholar]
  20. Ward N. , Staley J. T. , Fuerst J. A. , Giovannoni S. , Schlesner H. , Stackebrandt E. . ( 2006; ). The order Planctomycetales, including the genera Planctomyces, Pirellula, Gemmata and Isosphaera and the Candidatus genera Brocadia, Kuenenia and Scalindua . . In The Prokaryotes: a Handbook on the Biology of Bacteria, , 3rd edn., vol. 7, pp. 757–793. Edited by Dworkin M. , Falkow S. , Rosenberg E. , Schleifer K. H. , Stackebrandt E. . . New York:: Springer;. [CrossRef]
    [Google Scholar]
  21. Weisburg W. G. , Barns S. M. , Pelletier D. A. , Lane D. J. . ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173:, 697–703.[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.025924-0
Loading
/content/journal/ijsem/10.1099/ijs.0.025924-0
Loading

Data & Media loading...

Supplements

Supplementary figures 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error