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Abstract
An aerobic, pink-pigmented, budding bacterium, designated strain S26T, was isolated from an acidic Sphagnum peat bog of north-western Russia. Cells were non-motile and spherical, occurring singly, in pairs or in short chains, and were able to attach to surfaces by means of a holdfast material. Strain S26T was a moderately acidophilic, mesophilic organism capable of growth at pH 3.2–7.1 (optimum at pH 4.8–5.0) and at 4–33 °C (optimum at 20–26 °C). Most sugars, several organic acids and polyalcohols were the preferred growth substrates. The major fatty acids were C16 : 0, C18 : 1ω9c and C18 : 2ω6c,12c. The major neutral lipids were n-C31 : 9 hydrocarbon and squalene; the polar lipids were phosphatidylglycerol, phosphatidylcholine and components with an unknown structure. The DNA G+C content of strain S26T was 62.2 mol%. 16S rRNA gene sequence analysis showed that strain S26T is a member of the order Planctomycetales. Among taxonomically characterized representatives of this order, highest levels of 16S rRNA gene sequence similarity (95.1–95.2 %) were observed with strains of the non-filamentous, peat-inhabiting planctomycete Singulisphaera acidiphila. Strain S26T could be differentiated from Singulisphaera acidiphila based on pigmentation, significant differences in substrate utilization patterns, greater tolerance of acidic conditions and the presence of C16 : 1ω9c. Based on the data presented, strain S26T is considered to represent a novel species of the genus Singulisphaera, for which the name Singulisphaera rosea sp. nov. is proposed; the type strain is S26T ( = DSM 23044T = VKM B-2599T).
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Funding
- Molecular and Cell Biology’ of Russian Academy of Sciences
- Rosnauka project (Award 02.740.11.0023)