1887

Abstract

Six isolates of a facultatively anaerobic bacterium were recovered in culture from marine invertebrates and vertebrates, including packhorse lobster (), abalone ( sp.) and Atlantic salmon (), between 1994 and 2002. The bacteria were Gram-negative, rod-shaped and motile by means of more than one polar flagellum, oxidase-positive, catalase-positive and able to grow in the presence of 0.5–8.0 % NaCl (optimum 3.0–6.0 %) and at 10–37 °C (optimum 25–30 °C). On the basis of 16S rRNA gene sequence analysis and multilocus sequence analysis (MLSA) using five loci (2443 bp; , , , and ), the closest phylogenetic neighbours of strain TCFB 0772 were the type strains of (99.8 and 94.6 % similarity, respectively), (99.8 and 94.1 %), (99.4 and 88.8 %), (99.4 and 90.3 %), (99.2 and 94.4 %), (99.1 and 89.3 %) and (99.1 and 92.3 %). DNA–DNA hybridization confirmed that the six isolates constitute a unique taxon that is distinct from other known species of . In addition, this taxon can be readily differentiated phenotypically from other species. The six isolates therefore represent a novel species, for which the name sp. nov. is proposed; the novel species is represented by the type strain TCFB 0772 ( = JCM 16453  = LMG 25398) (DNA G+C content 45.9 mol%) and reference strains TCFB 1977 ( = JCM 16454) and TCFB 1000 ( = JCM 16455).

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2012-08-01
2019-10-20
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