1887

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Volume 61, Issue 5

gen. nov., sp. nov. and sp. nov., isolated from marine sand, deep-branching members of the phylum Free

Abstract

Two Gram-stain-negative, non-motile, non-spore-forming, rod-shaped bacterial strains, designated 3B-2 and 10AO, were isolated from a sand sample collected from the west coast of the Korean peninsula by using low-nutrient media, and their taxonomic positions were investigated in a polyphasic study. The strains did not grow on marine agar. They grew optimally at 30 °C and pH 6.5–7.5. Strains 3B-2 and 10AO shared 97.5 % 16S rRNA gene sequence similarity and mean level of DNA–DNA relatedness of 12 %. In phylogenetic trees based on 16S rRNA gene sequences, strains 3B-2 and 10AO, together with several uncultured bacterial clones, formed independent lineages within the evolutionary radiation encompassed by the phylum . Strains 3B-2 and 10AO contained MK-7 as the predominant menaquinone and iso-C and Cω5 as the major fatty acids. The DNA G+C contents of strains 3B-2 and 10AO were 42.8 and 44.6 mol%, respectively. Strains 3B-2 and 10AO exhibited very low levels of 16S rRNA gene sequence similarity (<85.0 %) to the type strains of recognized bacterial species. These data were sufficient to support the proposal that the novel strains should be differentiated from previously known genera of the phylum . On the basis of the data presented, we suggest that strains 3B-2 and 10AO represent two distinct novel species of a new genus, for which the names gen. nov., sp. nov. (the type species; type strain 3B-2  = KCTC 23018  = CCUG 58939) and sp. nov. (type strain 10AO  = KCTC 23019  = CCUG 58938) are proposed.

  • Published Online:
Funding
This study was supported by the:
  • 21C Frontier program of Microbial Genomics and Applications (Award MG05-0401-2-0)
  • Support and Application Project of Biological Resources (Award M10508050004-06N0805-00410)
  • Ministry of Education, Science and Technology (MEST) of the Republic of Korea
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Ohtaekwangia koreensis gen. nov., sp. nov. and Ohtaekwangia kribbensis sp. nov., isolated from marine sand, deep-branching members of the phylum Bacteroidetes
Int J Syst Evol Microbiol 61, 1066 (2011); https://doi.org/10.1099/ijs.0.025874-0
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