1887

Abstract

A bacterial strain, GW-9, which was isolated from groundwater in Korea, was subjected to a polyphasic taxonomic study using phenotypic characterization and phylogenetic and genetic methods. Phylogenetic analysis based on 16S rDNA sequences showed that strain GW-9 forms an evolutionary lineage within the radiation enclosing species and, in particular, a coherent cluster with . The cell-wall peptidoglycan type of strain GW-9 was based on -diaminopimelic acid as the diamino acid, indicating wall chemotype I. The predominant menaquinone was MK-8(H). Strain GW-9 had a cellular fatty acid profile containing straight-chain, branched, unsaturated and 10-methyl fatty acids. The major fatty acid was iso-C. The DNA G+C content of strain GW-9 was 73 mol%. The 16S rDNA of strain GW-9 was 99·2 % similar to that of the type strain of and 94·9–96·0 % similar to sequences of the type strains of other species. Differences in phenotypic characteristics and genetic distinctiveness indicate that strain GW-9 is separate from previously described species. Therefore, on the basis of the data presented, a novel species of the genus , sp. nov., is proposed. The type strain is strain GW-9 (=KCCM 41647=JCM 11813).

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2004-01-01
2021-10-24
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References

  1. Collins M. D., Cockcroft S., Wallbanks S. 1994; Phylogenetic analysis of a new ll-diaminopimelic acid-containing coryneform bacterium from herbage, Nocardioides plantarum sp. nov. Int J Syst Bacteriol 44:523–526 [CrossRef]
    [Google Scholar]
  2. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1993 phylip (phylogenetic inference package), version 3.5. Distributed by the author Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  6. Iizuka H., Komagata K. 1964; Microbiological studies on petroleum and natural gas. I. Determination of hydrocarbon-utilizing bacteria. J Gen Appl Microbiol 10:207–221 [CrossRef]
    [Google Scholar]
  7. Jensen H. L. 1934; Studies on saprophytic mycobacteria and corynebacteria. Proc Linn Soc N S W 5919–61
    [Google Scholar]
  8. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol 3 pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  9. Kluge A. G., Farris F. S. 1969; Quantitative phyletics and the evolution of anurans. Syst Zool 18:1–32 [CrossRef]
    [Google Scholar]
  10. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–206
    [Google Scholar]
  11. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  12. Lawson P. A., Collins M. D., Schumann P., Tindall B. J., Hirsch P., Labrenz M. 2000; New ll-diaminopimelic acid-containing actinomycetes from hypersaline, heliothermal and meromictic Antarctic Ekho Lake: Nocardioides aquaticus sp.nov. and Friedmanniella lacustris sp. nov. Syst Appl Microbiol 23:219–229 [CrossRef]
    [Google Scholar]
  13. Lechevalier M. P., Lechevalier H. A. 1970; A critical evaluation of the genera of aerobic actinomycetes. In The Actinomycetales pp  393–405 Edited by Prauser H. Jena: Gustav Fischer;
    [Google Scholar]
  14. Miller E. S., Woese C. R., Brenner S. 1991; Description of the erythromycin-producing bacterium Arthrobacter sp. strain NRRL B-3381 as Aeromicrobium erythreum gen. nov., sp. nov. Int J Syst Bacteriol 41:363–368 [CrossRef]
    [Google Scholar]
  15. Park Y.-H., Yoon J.-H., Shin Y. K., Suzuki K., Kudo T., Seino A., Kim H.-J., Lee J.-S., Lee S. T. 1999; Classification of ‘ Nocardioides fulvus ’ IFO 14399 and Nocardioides sp. ATCC 39419 in Kribbella gen. nov., as Kribbella flavida sp. nov. and Kribbella sandramycini sp. nov. Int J Syst Bacteriol 49:743–752 [CrossRef]
    [Google Scholar]
  16. Prauser H. 1976; Nocardioides , a new genus of the order Actinomycetales . Int J Syst Bacteriol 26:58–65 [CrossRef]
    [Google Scholar]
  17. Prauser H. 1989; Genus Nocardioides Prauser 1976. In Bergey's Manual of Systematic Bacteriology vol 4 pp  2371–2375 Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  18. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  19. Shirling E. B., Gottlieb D. 1966; Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16:313–340 [CrossRef]
    [Google Scholar]
  20. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  21. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  22. Urzì C., Salamone P., Schumann P., Stackebrandt E. 2000; Marmoricola aurantiacus gen. nov., sp. nov., a coccoid member of the family Nocardioidaceae isolated from a marble statue. Int J Syst Evol Microbiol 50:529–536 [CrossRef]
    [Google Scholar]
  23. Wang Y. M., Zhang Z. S., Xu X. L., Ruan J. S., Wang Y. 2001; Actinopolymorpha singaporensis gen. nov., sp. nov. a novel actinomycete from the tropical rainforest of Singapore. Int J Syst Evol Microbiol 51:467–473
    [Google Scholar]
  24. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  25. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  26. Yoon J.-H., Rhee S.-K., Lee J.-S., Park Y.-H., Lee S. T. 1997; Nocardioides pyridinolyticus sp. nov., a pyridine-degrading bacterium isolated from the oxic zone of an oil shale column. Int J Syst Bacteriol 47:933–938 [CrossRef]
    [Google Scholar]
  27. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
    [Google Scholar]
  28. Yoon J.-H., Cho Y.-G., Lee S. T., Suzuki K., Nakase T., Park Y.-H. 1999; Nocardioides nitrophenolicus sp. nov., a p -nitrophenol-degrading bacterium. Int J Syst Bacteriol 49:675–680 [CrossRef]
    [Google Scholar]
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