1887

Abstract

Twenty-two isolates from microbial mats in eastern Antarctic lakes showed similar fatty acid compositions and were investigated further using a polyphasic taxonomic approach. Repetitive extragenic palindromic DNA-PCR fingerprinting of the 22 strains revealed three groups, and DNA–DNA hybridizations between representatives showed more than 87 % DNA–DNA reassociation with each other. 16S rRNA gene sequence analysis placed two representative strains, LMG 21477 and LMG 21619, within the genus , with 95·1 % sequence similarity to , 95·0 % to , less than 95 % to other species and less than 90 % to representatives of other genera. The name sp. nov. is proposed, with LMG 21477 (=DSM 15343) as the type strain, and a description of the species is given on the basis of morphological, biochemical and physiological characteristics and fatty acid composition. The G+C content of the genomic DNA is 30·0–30·4 mol%.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02583-0
2003-09-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/53/5/ijs531241.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02583-0&mimeType=html&fmt=ahah

References

  1. Anacker R. L., Ordal E. J. 1955; Study of a bacteriophage infecting the myxobacterium Chondrococcus columnaris . J Bacteriol 70:738–741
    [Google Scholar]
  2. Barrow G. I., Feltham R. K. A. 1993 Cowan and Steel's Manual for the Identification of Medical Bacteria , 3rd edn. Cambridge: Cambridge University Press;
    [Google Scholar]
  3. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. 1996; Cutting a Gordian knot: emended classification and description of the genus Flavobacterium , emended description of the family Flavobacteriaceae , and proposal of Flavobacterium hydatis nom. nov. (basonym) Cytophaga aquatilis Strohl and Tait 1978. Int J Syst Bacteriol 46:128–148 [CrossRef]
    [Google Scholar]
  4. Bernardet J.-F., Nakagawa Y., Holmes B. 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [CrossRef]
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  6. Humphry D. R., George A., Black G. W., Cummings S. P. 2001; Flavobacterium frigidarium sp. nov., an aerobic, psychrophilic, xylanolytic and laminarinolytic bacterium from Antarctica. Int J Syst Evol Microbiol 51:1235–1243
    [Google Scholar]
  7. Kirchman D. L. 2002; The ecology of Cytophaga-Flavobacterium in aquatic environments. FEMS Microbiol Ecol 39:91–100
    [Google Scholar]
  8. McCammon S. A., Bowman J. P. 2000; Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov. and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [ Flavobacterium ] salegens as Salegentibacter salegens gen. nov., comb. nov. Int J Syst Evol Microbiol 501055–1063 [CrossRef]
    [Google Scholar]
  9. Mergaert J., Verhelst A., Cnockaert M. C., Tan T.-L., Swings J. 2001; Characterization of facultative oligotrophic bacteria from polar seas by analysis of their fatty acids and 16S rDNA sequences. Syst Appl Microbiol 24:98–107 [CrossRef]
    [Google Scholar]
  10. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  11. Paton A. M. 1959; An improved method for preparing pectate gels. Nature 183:1812–1813
    [Google Scholar]
  12. Pitcher D. G., Saunders N. A., Owen R. J. 1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151–156 [CrossRef]
    [Google Scholar]
  13. Rademaker J. L. W., de Bruijn F. J. 1997; Characterization and classification of microbes by rep-PCR genomic fingerprinting and computer assisted pattern analysis. In DNA Markers: ProtocolsApplications and Overviews pp 151–171Edited by Caetano-Anollés G., Gresshoff P. M. New York: Wiley;
    [Google Scholar]
  14. Rademaker J. L. W., Hoste B., Louws F. J., Kersters K., Swings J., Vauterin L., Vauterin P., de Bruijn F. J. 2000; Comparison of AFLP and rep-PCR genomic fingerprinting with DNA–DNA homology studies: Xanthomonas as a model system. Int J Syst Evol Microbiol 50:665–677 [CrossRef]
    [Google Scholar]
  15. Reichenbach H. 1989; Order I. Cytophagales Leadbetter 1974, 99AL. In Bergey's Manual of Systematic Bacteriology vol 3 pp 2011–2013Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  16. Reichenbach H., Dworkin M. 1981; Introduction to the gliding bacteria. In The Prokaryotes vol 1 pp 315–327Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel. Berlin: Springer;
    [Google Scholar]
  17. Saitou N., Nei M. 1987; The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  18. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  19. Van Trappen S., Mergaert J., Swings J. 2001; Genomic diversity of heterotrophic bacteria in microbial mats from Antarctic lakes. In Abstracts of the 8th SCAR International Biology Symposium: Antarctic Biology in a Global Context Amsterdam: Abstract no. S5O09
    [Google Scholar]
  20. Van Trappen S., Mergaert J., Van Eygen S., Dawyndt P., Cnockaert M. C., Swings J. 2002; Diversity of 746 heterotrophic bacteria isolated from microbial mats from ten Antarctic lakes. Syst Appl Microbiol 25:603–610 [CrossRef]
    [Google Scholar]
  21. Versalovic J., Koeuth T., Lupski J. R. 1991; Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting in bacterial genomes. Nucleic Acids Res 19:6823–6831 [CrossRef]
    [Google Scholar]
  22. Versalovic J., Schneider M., de Bruijn F. J., Lupski J. R. 1994; Genomic fingerprinting of bacteria using repetitive sequence-based polymerase chain reaction. Methods Mol Cell Biol 5:25–40
    [Google Scholar]
  23. Wayne L. G., Brenner D. J., Colwell R. R.9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  24. West P. A., Colwell R. R. 1984; Identification and classification of the Vibrionaceae – an overview. In Vibrios in the Environment pp 285–363Edited by Colwell R. R. H. G. New York: Wiley;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02583-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02583-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error