1887

Abstract

A Gram-reaction-positive, non-motile actinobacterium, designated strain 210121, was isolated from rhizosphere soil of the mangrove fern . 16S rRNA gene sequence analysis showed that the isolate belonged to the genus . DNA–DNA relatedness values between strain 210121 and the type strains of the three recognized species of the genus were below the 70 % threshold recommended for distinguishing bacterial genomic species. The novel isolate contained glutamic acid, glycine, alanine and -Apm as cell-wall amino acids, indicating peptidoglycan type A1γ. The characteristic whole-cell sugars were xylose, ribose, glucose and mannose. The predominant menaquinones were MK-9(H), MK-9(H) and MK-9(H). The major fatty acids were iso-C (30.9 %), C (23.0 %), anteiso-C (14.9 %) and iso-C (12.3 %). The phospholipid profile comprised phosphatidylethanolamine, phosphatidylinositol mannosides and phospholipids of unknown structure containing glucosamine. The G+C content of the DNA was 70.3 mol%. On the basis of the phenotypic and genotypic data, strain 210121 ( = CGMCC 4.5593  = DSM 45427) represents a novel species of the genus , for which the name sp. nov., is proposed. An emended description of the genus is also proposed.

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2011-10-01
2019-10-19
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Neighbour-joining phylogenetic tree based on almost-complete 16S rRNA gene sequences, showing the relationships between strain 210121 , the three type species of the genus and representatives of genera in the family . [PDF](31KB)

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Light micrograph (×1000) of strain 210121 grown on M8 medium at 28 °C for 21 days. Red arrowhead indicates a spore chain. Bar, 1400 μm.

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