1887

Abstract

A beige-coloured, Gram-staining negative, aerobic, non-motile, moderately thermophilic, rod-shaped bacterium, CC-MHSW-1, was isolated on Marine Agar 2216 from a water sample from a coastal hot spring on Green Island (Lutao), located off Taituang, Taiwan. 16S rRNA gene sequence analysis demonstrated that the novel strain shared <94 % sequence similarity with members of the genera and . Ubiquinone (Q-10) was the major respiratory quinone and Cω7 was the predominant fatty acid. The predominant polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and unidentified phospholipids and aminolipids. The DNA G+C content of strain CC-MHSW-1 was 64.7 mol%. On the basis of 16S rRNA gene sequence analysis and the chemotaxonomic and physiological data, strain CC-MHSW-1 represents a new genus and species in the family for which the name gen. nov., sp. nov., is proposed. The type strain of the type species is CC-MHSW-1 ( = JCM 15068 = CCM 7767).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.025817-0
2011-07-01
2019-12-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/7/1544.html?itemId=/content/journal/ijsem/10.1099/ijs.0.025817-0&mimeType=html&fmt=ahah

References

  1. Bernardet J.-F. , Nakagawa Y. , Holmes B. . Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef].[PubMed]
    [Google Scholar]
  2. Bruns A. , Rohde M. , Berthe-Corti L. . ( 2001; ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51:, 1997–2006.[PubMed] [CrossRef]
    [Google Scholar]
  3. Cho J.-C. , Giovannoni S. J. . ( 2004; ). Oceanicola granulosus gen. nov., sp. nov. and Oceanicola batsensis sp. nov., poly-β-hydroxybutyrate-producing marine bacteria in the order ‘Rhodobacterales’. . Int J Syst Evol Microbiol 54:, 1129–1136. [CrossRef].[PubMed]
    [Google Scholar]
  4. Chung Y. C. , Kobayashi T. , Kanai H. , Akiba T. , Kudo T. . ( 1995; ). Purification and properties of extracellular amylase from the hyperthermophilic archaeon Thermococccus profundus DT5432. . Appl Environ Microbiol 61:, 1502–1506.[PubMed]
    [Google Scholar]
  5. Collins M. D. , Jones D. . ( 1980; ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. . J Appl Bacteriol 48:, 459–470.[CrossRef]
    [Google Scholar]
  6. Collins M. D. , Goodfellow M. , Minnikin D. E. . ( 1979; ). Isoprenoid quinones in the classification of coryneform and related bacteria. . J Gen Microbiol 110:, 127–136.[PubMed] [CrossRef]
    [Google Scholar]
  7. Cowan S. T. , Steel K. J. . ( 1965; ). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
    [Google Scholar]
  8. Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  9. Groth I. , Schumann P. , Weiss N. , Martin K. , Rainey F. A. . ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. . Int J Syst Bacteriol 46:, 234–239. [CrossRef].[PubMed]
    [Google Scholar]
  10. Gu J. , Guo B. , Wang Y.-N. , Yu S.-L. , Inamori R. , Qu R. , Ye Y.-G. , Wu X.-L. . ( 2007; ). Oceanicola nanhaiensis sp. nov., isolated from sediments of the South China Sea. . Int J Syst Evol Microbiol 57:, 157–160. [CrossRef].[PubMed]
    [Google Scholar]
  11. Kämpfer P. , Kroppenstedt R. M. . ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42:, 989–1005. [CrossRef]
    [Google Scholar]
  12. Kumar S. , Tamura K. , Jakobsen I. B. , Nei M. . ( 2001; ). mega2: molecular evolutionary genetics analysis software. . Bioinformatics 17:, 1244–1245. [CrossRef].[PubMed]
    [Google Scholar]
  13. Labrenz M. , Lawson P. A. , Tindall B. J. , Collins M. D. , Hirsch P. . ( 2005; ). Roseisalinus antarcticus gen. nov., sp. nov., a novel aerobic bacteriochlorophyll a-producing α-proteobacterium isolated from hypersaline Ekho Lake, Antarctica. . Int J Syst Evol Microbiol 55:, 41–47. [CrossRef].[PubMed]
    [Google Scholar]
  14. Lányí B. . ( 1987; ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19:, 1–67. [CrossRef]
    [Google Scholar]
  15. Lee K. , Choo Y. J. , Giovannoni S. J. , Cho J. C. . ( 2007; ). Maritimibacter alkaliphilus gen. nov., sp. nov., a genome-sequenced marine bacterium of the Roseobacter clade in the order Rhodobacterales . . Int J Syst Evol Microbiol 57:, 1653–1658. [CrossRef].[PubMed]
    [Google Scholar]
  16. Leifson E. . ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85:, 1183–1184.[PubMed]
    [Google Scholar]
  17. Lin K.-Y. , Sheu S.-Y. , Chang P.-S. , Cho J.-C. , Chen W.-M. . ( 2007; ). Oceanicola marinus sp. nov., a marine alphaproteobacterium isolated from seawater collected off Taiwan. . Int J Syst Evol Microbiol 57:, 1625–1629. [CrossRef].[PubMed]
    [Google Scholar]
  18. Ludwig W. , Strunk O. , Westram R. , Richter L. , Meier H. , Yadhukumar , Buchner A. , Lai T. , Steppi S. et al. ( 2004; ). arb: a software environment for sequence data. . Nucleic Acids Res 32:, 1363–1371.[CrossRef]
    [Google Scholar]
  19. Martínez-Cánovas M. J. , Quesada E. , Martínez-Checa F. , del Moral A. , Béjar V. . ( 2004; ). Salipiger mucescens gen. nov., sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium isolated from hypersaline soil, belonging to the α-Proteobacteria . . Int J Syst Evol Microbiol 54:, 1735–1740. [CrossRef].[PubMed]
    [Google Scholar]
  20. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the GC content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  21. Miller P. H. , Wiggs L. S. , Miller J. M. . ( 1995; ). Evaluation of AnaeroGen system for growth of anaerobic bacteria. . J Clin Microbiol 33:, 2388–2391.[PubMed]
    [Google Scholar]
  22. Minnikin D. E. , Collins M. D. , Goodfellow M. . ( 1979; ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47:, 87–95.[CrossRef]
    [Google Scholar]
  23. Olsen G. J. , Matsuda H. , Hagstrom R. , Overbeek R. . ( 1994; ). fastDNAmL: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood. . Comput Appl Biosci 10:, 41–48.
    [Google Scholar]
  24. Pruesse E. , Quast C. , Knittel K. , Fuchs B. M. , Ludwig W. , Peplies J. , Glöckner F. O. . ( 2007; ). silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb . . Nucleic Acids Res 35:, 7188–7196.[CrossRef]
    [Google Scholar]
  25. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. . USFCC Newsl 20:, 16.
    [Google Scholar]
  26. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  27. Suzuki T. , Muroga Y. , Takahama M. , Nishimura Y. . ( 1999; ). Roseivivax halodurans gen. nov., sp. nov. and Roseivivax halotolerans sp. nov., aerobic bacteriochlorophyll-containing bacteria isolated from a saline lake. . Int J Syst Bacteriol 49:, 629–634. [CrossRef].[PubMed]
    [Google Scholar]
  28. Takai K. , Miyazaki M. , Hirayama H. , Nakagawa S. , Querellou J. , Godfroy A. . ( 2009; ). Isolation and physiological characterization of two novel, piezophilic, thermophilic chemolithoautotrophs from a deep-sea hydrothermal vent chimney. . Environ Microbiol 11:, 1983–1997. [CrossRef].[PubMed]
    [Google Scholar]
  29. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef].[PubMed]
    [Google Scholar]
  30. Yoon J.-H. , Kang S.-J. , Oh T.-K. . ( 2007; ). Donghicola eburneus gen. nov., sp. nov., isolated from seawater of the East Sea in Korea. . Int J Syst Evol Microbiol 57:, 73–76. [CrossRef].[PubMed]
    [Google Scholar]
  31. Yoon J.-H. , Kang S.-J. , Lee J.-S. , Oh T.-K. . ( 2009; ). Lutimaribacter saemankumensis gen. nov., sp. nov., isolated from a tidal flat of the Yellow Sea. . Int J Syst Evol Microbiol 59:, 48–52. [CrossRef].[PubMed]
    [Google Scholar]
  32. Young C.-C. , Kämpfer P. , Shen F.-T. , Lai W.-A. , Arun A. B. . ( 2005; ). Chryseobacterium formosense sp. nov., isolated from the rhizosphere of Lactuca sativa L. (garden lettuce). . Int J Syst Evol Microbiol 55:, 423–426. [CrossRef].[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.025817-0
Loading
/content/journal/ijsem/10.1099/ijs.0.025817-0
Loading

Data & Media loading...

Supplements

vol. , part 7, pp. 1544–1548

Two-dimensional TLC of polar lipid extracts from strain CC-MHSW-1 , stained with molybdatophosphoric acid. PC, phosphatidylcholine; PG, phosphatidylglycerol, PE, phosphatidylethanolamine, DPG, diphosphatidylglycerol; PL1–PL6, unknown phospholipids, AL, aminolipids, GL, unknown glycolipid.



IMAGE

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error