1887

Abstract

Strains of fluorescent pseudomonads, isolated from the phyllosphere of grasses, were analysed by a polyphasic approach in order to clarify their interspecific position. Classification on the basis of ribotyping revealed six genotypes; four of these, which could be differentiated clearly from each other and from species with validly published names on the basis of phenotypic features, were chosen for detailed phylogenetic analysis. DNA–DNA hybridization studies among representative strains of the four genotypes and closely related species, determined by comparison of 16S rDNA sequences, showed that three of the studied ribotypes represented novel species. Two of them were related to mainly saprophytic fluorescent pseudomonads and could be easily distinguished by a negative arginine dihydrolase reaction. One ribotype, also characterized by a negative arginine dihydrolase reaction, was closely related to potentially plant-pathogenic fluorescent pseudomonads and differed in certain phenotypic features from its phylogenetic neighbours. As a consequence of the phenotypic and phylogenetic analyses, sp. nov. (type strain: P 513/19=DSM 14937=LMG 21464), sp. nov. (type strain: P 527/13=DSM 14936=LMG 21465) and sp. nov. (type strain: P 538/23=DSM 14939=LMG 21466) are proposed.

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2003-09-01
2019-10-23
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References

  1. Anzai, Y., Kim, H., Park, J.-Y., Wakabayashi, H. & Oyaizu, H. ( 2000; ). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50, 1563–1589.[CrossRef]
    [Google Scholar]
  2. Baïda, N., Yazourh, A., Singer, E. & Izard, D. ( 2002; ). Pseudomonas grimontii sp. nov. Int J Syst Evol Microbiol 52, 1497–1503.[CrossRef]
    [Google Scholar]
  3. Behrendt, U. ( 2001; ). Der Einfluß differenzierter Bewirtschaftungsintensität von Niedermoorgrünland auf die Entwicklung von Mikroorganismen-Gesellschaften in der Phyllosphäre von Gräsern. ZALF-Bericht no. 45. Müncheberg: Zentrum für Agrarlandschafts- und Landnutzungsforschung (ZALF) (in German).
  4. Behrendt, U., Ulrich, A., Schumann, P., Erler, W., Burghardt, J. & Seyfarth, W. ( 1999; ). A taxonomic study of bacteria isolated from grasses: a proposed new species Pseudomonas graminis sp. nov. Int J Syst Bacteriol 49, 297–308.[CrossRef]
    [Google Scholar]
  5. Billing, E. ( 1970; ). Pseudomonas viridiflava (Burkholder, 1930; Clara 1934). J Appl Bacteriol 33, 492–500.[CrossRef]
    [Google Scholar]
  6. Chun, J. & Goodfellow, M. ( 1995; ). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45, 240–245.[CrossRef]
    [Google Scholar]
  7. Coroler, L., Elomari, M., Hoste, B., Gillis, M., Izard, D. & Leclerc, H. ( 1996; ). Pseudomonas rhodesiae sp. nov., a new species isolated from natural mineral waters. Syst Appl Microbiol 19, 600–607.[CrossRef]
    [Google Scholar]
  8. Dabboussi, F., Hamze, M., Elomari, M., Verhille, S., Baida, N., Izard, D. & Leclerc, H. ( 1999; ). Taxonomic study of bacteria isolated from Lebanese spring waters: proposal for Pseudomonas cedrella sp. nov. and P. orientalis sp. nov. Res Microbiol 150, 303–316.[CrossRef]
    [Google Scholar]
  9. Delorme, S., Lemanceau, P., Christen, R., Corberand, T., Meyer, J.-M. & Gardan, L. ( 2002; ). Pseudomonas lini sp. nov., a novel species from bulk and rhizospheric soils. Int J Syst Evol Microbiol 52, 513–523.
    [Google Scholar]
  10. Elomari, M., Coroler, L., Hoste, B., Gillis, M., Izard, D. & Leclerc, H. ( 1996; ). DNA relatedness among Pseudomonas strains isolated from natural mineral waters and proposal of Pseudomonas veronii sp. nov. Int J Syst Bacteriol 46, 1138–1144.[CrossRef]
    [Google Scholar]
  11. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  12. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5c. Department of Genetics, University of Washington, Seattle, USA.
  13. Gardan, L., Cottin, S., Bollet, C. & Hunault, G. ( 1991; ). Phenotypic heterogeneity of Pseudomonas syringae van Hall. Res Microbiol 142, 995–1003.[CrossRef]
    [Google Scholar]
  14. Gardan, L., Bollet, C., Abu Ghorrah, M., Grimont, F. & Grimont, P. A. D. ( 1992; ). DNA relatedness among the pathovar strains of Pseudomonas syringae subsp. savastanoi Janse (1982) and proposal of Pseudomonas savastanoi sp. nov. Int J Syst Bacteriol 42, 606–612.[CrossRef]
    [Google Scholar]
  15. Gardan, L., Shafik, H., Belouin, S., Broch, R., Grimont, F. & Grimont, P. A. D. ( 1999; ). DNA relatedness among the pathovars of Pseudomonas syringae and description of Pseudomonas tremae sp. nov. and Pseudomonas cannabina sp. nov. (ex Sutic and Dowson 1959). Int J Syst Bacteriol 49, 469–478.[CrossRef]
    [Google Scholar]
  16. Gardan, L., Bella, P., Meyer, J.-M., Christen, R., Rott, P., Achouak, W. & Samson, R. ( 2002; ). Pseudomonas salomonii sp. nov., pathogenic on garlic, and Pseudomonas palleroniana sp. nov., isolated from rice. Int J Syst Evol Microbiol 52, 2065–2074.[CrossRef]
    [Google Scholar]
  17. Hildebrand, D. C., Schroth, M. N. & Sands, D. C. ( 1994; ). Pseudomonas. In Laboratory Guide for Identification of Plant Pathogenic Bacteria, pp. 60–80. Edited by N. W. Schaad. St Paul, MN: American Phytopathological Society.
  18. Holt, J. G., Krieg, N. R., Sneath, P. H. A., Staley, J. T. & Williams, S. T. (editors) ( 1994; ). Bergey's Manual of Determinative Bacteriology, 9th edn. Baltimore: Williams & Wilkins.
  19. Hu, F.-P., Young, J. M. & Triggs, C. M. ( 1991; ). Numerical analysis and determinative tests for nonfluorescent plant-pathogenic Pseudomonas spp. and genomic analysis and reclassification of species related to Pseudomonas avenae Manns 1909. Int J Syst Bacteriol 41, 516–525.[CrossRef]
    [Google Scholar]
  20. Hugh, R. & Leifson, E. ( 1953; ). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram-negative bacteria. J Bacteriol 66, 24–26.
    [Google Scholar]
  21. Ivanova, E. P., Gorshkova, N. M., Sawabe, T. & 8 other authors ( 2002; ). Pseudomonas extremorientalis sp. nov., isolated from a drinking water reservoir. Int J Syst Evol Microbiol 52, 2113–2120.[CrossRef]
    [Google Scholar]
  22. Janse, J. D., Rossi, P., Angelucci, L., Scortichini, M., Derks, J. H. J., Akkermans, A. D. L., de Vrijer, R. & Psallidas, P. G. ( 1996; ). Reclassification of Pseudomonas syringae pv. avellanae as Pseudomonas avellanae (spec. nov.), the bacterium causing canker of hazelnut (Corylus avellana L.). Syst Appl Microbiol 19, 589–595.[CrossRef]
    [Google Scholar]
  23. King, E. O., Ward, M. K. & Raney, D. E. ( 1954; ). Two simple media for the demonstration of pyocyanin and fluorescein. J Lab Clin Med 44, 301–307.
    [Google Scholar]
  24. Martin, K., Schumann, P., Rainey, F. A., Schuetze, B. & Groth, I. ( 1997; ). Janibacter limosus gen. nov., sp. nov., a new actinomycete with meso-diaminopimelic acid in the cell wall. Int J Syst Bacteriol 47, 529–534.[CrossRef]
    [Google Scholar]
  25. Munsch, P., Alatossava, T., Marttinen, N., Meyer, J.-M., Christen, R. & Gardan, L. ( 2002; ). Pseudomonas costantinii sp. nov., another causal agent of brown blotch disease, isolated from cultivated mushroom sporophores in Finland. Int J Syst Evol Microbiol 52, 1973–1983.[CrossRef]
    [Google Scholar]
  26. Psallidas, P. G. & Panagopoulos, C. G. ( 1975; ). A new bacteriosis of almond caused by Pseudomonas amygdali sp. nov. Ann Inst Phytopathol Benaki 11, 94–108.
    [Google Scholar]
  27. Rudolph, K., Roy, M. A., Sasser, M., Stead, D. E., Davis, D. E., Swings, M. & Gosselé, F. ( 1990; ). Isolation of bacteria. In Methods in Phytobacteriology, pp. 43–86. Edited by Z. Klement, K. Rudolph & D. C. Sands. Budapest: Akademiai Kiado.
  28. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  29. Sands, D. C. ( 1990; ). Physiological criteria – determinative tests. In Methods in Phytobacteriology, pp. 133–143. Edited by Z. Klement, K. Rudolph & D. C. Sands. Budapest: Akademiai Kiado.
  30. Schroth, M. N., Hildebrand, D. C. & Panopoulos, N. ( 1992; ). Phytopathogenic pseudomonads and related plant-associated pseudomonads. In The Prokaryotes, pp. 3104–3131. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K.-H. Schleifer. New York: Springer-Verlag.
  31. Sikorski, J., Stackebrandt, E. & Wackernagel, W. ( 2001; ). Pseudomonas kilonensis sp. nov., a bacterium isolated from agricultural soil. Int J Syst Evol Microbiol 51, 1549–1555.
    [Google Scholar]
  32. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  33. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J. ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703.
    [Google Scholar]
  34. Young, J. M. ( 1970; ). Drippy gill: a bacterial disease of cultivated mushrooms caused by Pseudomonas agarici n. sp. N Z J Agric Res 13, 977–990.[CrossRef]
    [Google Scholar]
  35. Young, J. M. & Triggs, C. M. ( 1994; ). Evaluation of determinative tests for pathovars of Pseudomonas syringae van Hall 1902. J Appl Bacteriol 77, 195–207.[CrossRef]
    [Google Scholar]
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