1887

Abstract

A Gram-negative, aerobic, short rod-shaped and non-motile bacterium, strain A-1, was isolated from a saline soil contaminated with crude oil in Xianhe, Shangdong Province, China. Strain A-1 formed yellow colonies, was moderately halophilic and grew with 0.05–27.5 % (w/v) total salts (optimum 5–8 %), at 10–42 °C (optimum 30 °C) and at pH 5.5–9.0 (optimum pH 7.2). The dominant fatty acids (>5 %) were C, summed feature 3 (comprising Cω7 and/or iso-C 2-OH), Cω7, C cyclo ω8 and C 3-OH and the predominant ubiquinone was Q-9. The genomic DNA G+C content was 67.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain A-1 belonged to the genus in the class . The closest relatives were YIM 91125 (97.7 % 16S rRNA gene sequence similarity), LMG 20969 (95.6 %), AAP (95.5 %) and BH843 (95.2 %). DNA–DNA relatedness between strain A-1 and CCTCC AB 206093 was 27±3 %. On the basis of phenotypic, chemotaxonomic and phylogenetic features, strain A-1 should be placed in the genus as a representative of a novel species. The name sp. nov. is proposed, with strain A-1 ( = CGMCC 1.6848  = JCM 14849) as the type strain.

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2012-01-01
2019-10-20
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References

  1. Acinas S. G. , Antón J. , Rodríguez-Valera F. . ( 1999; ). Diversity of free-living and attached bacteria in offshore Western Mediterranean waters as depicted by analysis of genes encoding 16S rRNA. . Appl Environ Microbiol 65:, 514–522.[PubMed]
    [Google Scholar]
  2. Arahal D. R. , Ludwig W. , Schleifer K. H. , Ventosa A. . ( 2002; ). Phylogeny of the family Halomonadaceae based on 23S and 165 rDNA sequence analyses. . Int J Syst Evol Microbiol 52:, 241–249.[PubMed]
    [Google Scholar]
  3. Arahal D. R. , Vreeland R. H. , Litchfield C. D. , Mormile M. R. , Tindall B. J. , Oren A. , Bejar V. , Quesada E. , Ventosa A. . ( 2007; ). Recommended minimal standards for describing new taxa of the family Halomonadaceae . . Int J Syst Evol Microbiol 57:, 2436–2446. [CrossRef] [PubMed]
    [Google Scholar]
  4. Arenas M. , Bañón P. I. , Copa-Patiño J. L. , Sánchez-Porro C. , Ventosa A. , Soliveri J. . ( 2009; ). Halomonas ilicicola sp. nov., a moderately halophilic bacterium isolated from a saltern. . Int J Syst Evol Microbiol 59:, 578–582. [CrossRef] [PubMed]
    [Google Scholar]
  5. Baron E. J. , Finegold S. M. . ( 1990; ). Bailey and Scott’s Diagnostic Microbiology, , 8th edn.. St Louis:: Mosby;.
    [Google Scholar]
  6. Cabrera A. , Aguilera M. , Fuentes S. , Incerti C. , Russell N. J. , Ramos-Cormenzana A. , Monteoliva-Sánchez M. . ( 2007; ). Halomonas indalinina sp. nov., a moderately halophilic bacterium isolated from a solar saltern in Cabo de Gata, Almeria, southern Spain. . Int J Syst Evol Microbiol 57:, 376–380. [CrossRef] [PubMed]
    [Google Scholar]
  7. Callies E. , Mannheim W. . ( 1978; ). Classification of the Flavobacterium-Cytophaga complex on the basis of respiratory quinones and fumarate respiration. . Int J Syst Bacteriol 28:, 14–19. [CrossRef]
    [Google Scholar]
  8. de la Haba R. R. , Arahal D. R. , Márquez M. C. , Ventosa A. . ( 2010; ). Phylogenetic relationships within the family Halomonadaceae based on comparative 23S and 16S rRNA gene sequence analysis. . Int J Syst Evol Microbiol 60:, 737–748. [CrossRef] [PubMed]
    [Google Scholar]
  9. Dobson S. J. , Franzmann P. D. . ( 1996; ). Unification of the genera Deleya (Baumann et al. 1983), Halomonas (Vreeland et al. 1980), and Halovibrio (Fendrich 1988) and the species Paracoccus halodenitrificans (Robinson and Gibbons 1952) in to a single genus, Halomonas, and placement of the genus Zymobacter in the family Halomonadaceae . . Int J Syst Bacteriol 46:, 550–558. [CrossRef]
    [Google Scholar]
  10. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  11. Franzmann P. D. , Wehmeyer U. , Stackebrandt E. . ( 1988; ). Halomonadaceae fam. nov., a new family of the class Proteobacteria to accommodate the genera Halomonas and Deleya . . Syst Appl Microbiol 11:, 16–19.[CrossRef]
    [Google Scholar]
  12. García M. T. , Mellado E. , Ostos J. C. , Ventosa A. . ( 2004; ). Halomonas organivorans sp. nov., a moderate halophile able to degrade aromatic compounds. . Int J Syst Evol Microbiol 54:, 1723–1728. [CrossRef] [PubMed]
    [Google Scholar]
  13. Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  14. Heyrman J. , Balcaen A. , De Vos P. , Swings J. . ( 2002; ). Halomonas muralis sp. nov., isolated from microbial biofilms colonizing the walls and murals of the Saint-Catherine chapel (Castle Herberstein, Austria). . Int J Syst Evol Microbiol 52:, 2049–2054. [CrossRef] [PubMed]
    [Google Scholar]
  15. Ivanova E. P. , Kiprianova E. A. , Mikhailov V. V. , Levanova G. F. , Garagulya A. D. , Gorshkova N. M. , Vysotskii M. V. , Nicolau D. V. , Yumoto N. . & other authors ( 1998; ). Phenotypic diversity of Pseudoalteromonas citrea from different marine habitats and emendation of the description. . Int J Syst Bacteriol 48:, 247–256. [CrossRef] [PubMed]
    [Google Scholar]
  16. Jeon C. O. , Lim J.-M. , Lee J. R. , Lee G. S. , Park D.-J. , Lee J.-C. , Oh H.-W. , Kim C.-J. . ( 2007; ). Halomonas kribbensis sp. nov., a novel moderately halophilic bacterium isolated from a solar saltern in Korea. . Int J Syst Evol Microbiol 57:, 2194–2198. [CrossRef] [PubMed]
    [Google Scholar]
  17. Kluge A. G. , Farris F. S. . ( 1969; ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18:, 1–32. [CrossRef]
    [Google Scholar]
  18. Komagata K. , Suzuki K. . ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  19. Kumar S. , Nei M. , Dudley J. , Tamura K. . ( 2008; ). mega: a biologist-centric software for evolutionary analysis of DNA and protein sequences. . Brief Bioinform 9:, 299–306. [CrossRef] [PubMed]
    [Google Scholar]
  20. Lányí B. . ( 1987; ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19:, 1–67. [CrossRef]
    [Google Scholar]
  21. Larkin M. A. , Blackshields G. , Brown N. P. , Chenna R. , McGettigan P. A. , McWilliam H. , Valentin F. , Wallace I. M. , Wilm A. . & other authors ( 2007; ). clustal w and clustal_x version 2.0. . Bioinformatics 23:, 2947–2948. [CrossRef] [PubMed]
    [Google Scholar]
  22. Li J. , Chen C. , Zhao G.-Z. , Klenk H.-P. , Pukall R. , Zhang Y.-Q. , Tang S.-K. , Li W.-J. . ( 2009; ). Description of Dietzia lutea sp. nov., isolated from a desert soil in Egypt. . Syst Appl Microbiol 32:, 118–123. [CrossRef] [PubMed]
    [Google Scholar]
  23. Margesin R. , Schinner F. . ( 2001; ). Potential of halotolerant and halophilic microorganisms for biotechnology. . Extremophiles 5:, 73–83. [CrossRef] [PubMed]
    [Google Scholar]
  24. Marmur J. . ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  25. Martínez-Cánovas M. J. , Quesada E. , Llamas I. , Béjar V. . ( 2004; ). Halomonas ventosae sp. nov., a moderately halophilic, denitrifying, exopolysaccharide-producing bacterium. . Int J Syst Evol Microbiol 54:, 733–737. [CrossRef] [PubMed]
    [Google Scholar]
  26. Mata J. A. , Martínez-Cánovas J. , Quesada E. , Béjar V. . ( 2002; ). A detailed phenotypic characterisation of the type strains of Halomonas species. . Syst Appl Microbiol 25:, 360–375. [CrossRef] [PubMed]
    [Google Scholar]
  27. Nieto J. J. , Vargas C. , Ventosa A. . ( 2000; ). Osmoprotection mechanisms in the moderately halophilic bacterium Halomonas elongata . . In Recent Research Developments in Microbiology, vol. 4, pp. 43–54. Edited by Pandalai S. G. . . Trivandrum, India:: Research Signpost;.
    [Google Scholar]
  28. Okamoto T. , Taguchi H. , Nakamura K. , Ikenaga H. , Kuraishi H. , Yamasato K. . ( 1993; ). Zymobacter palmae gen. nov., sp. nov., a new ethanol-fermenting peritrichous bacterium isolated from palm sap. . Arch Microbiol 160:, 333–337. [CrossRef] [PubMed]
    [Google Scholar]
  29. Pettersson B. , de Silva S. K. , Uhlén M. , Priest F. G. . ( 2000; ). Bacillus siralis sp. nov., a novel species from silage with a higher order structural attribute in the 16S rRNA genes. . Int J Syst Evol Microbiol 50:, 2181–2187. [CrossRef] [PubMed]
    [Google Scholar]
  30. Quesada E. , Bejar V. , Valderrama M. J. , Ramos-Cormenzana A. . ( 1987; ). Growth characteristics and salt requirement of Deleya halophila in a defined medium. . Curr Microbiol 16:, 21–25. [CrossRef]
    [Google Scholar]
  31. Romano I. , Nicolaus B. , Lama L. , Manca M. C. , Gambacorta A. . ( 1996; ). Characterization of a haloalkalophilic strictly aerobic bacterium, isolated from Pantelleria island. . Syst Appl Microbiol 19:, 326–333. [CrossRef]
    [Google Scholar]
  32. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  33. Sánchez-Porro C. , Martín S. , Mellado E. , Ventosa A. . ( 2003; ). Diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes. . J Appl Microbiol 94:, 295–300. [CrossRef] [PubMed]
    [Google Scholar]
  34. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  35. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  36. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  37. Ventosa A. , Nieto J. J. , Oren A. . ( 1998; ). Biology of moderately halophilic aerobic bacteria. . Microbiol Mol Biol Rev 62:, 504–544.[PubMed]
    [Google Scholar]
  38. Vreeland R. H. , Litchfield C. D. , Martin E. L. , Elliot E. . ( 1980; ). Halomonas elongata, a new genus and species of extremely salt-tolerant bacteria. . Int J Syst Bacteriol 30:, 485–495. [CrossRef]
    [Google Scholar]
  39. Wang Y. , Tang S.-K. , Lou K. , Mao P.-H. , Jin X. , Jiang C.-L. , Xu L.-H. , Li W.-J. . ( 2008; ). Halomonas lutea sp. nov., a moderately halophilic bacterium isolated from a salt lake. . Int J Syst Evol Microbiol 58:, 2065–2069. [CrossRef] [PubMed]
    [Google Scholar]
  40. Wang Y. , Tang S.-K. , Lou K. , Lee J.-C. , Jeon C. O. , Xu L.-H. , Kim C.-J. , Li W.-J. . ( 2009; ). Aidingimonas halophila gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt lake. . Int J Syst Evol Microbiol 59:, 3088–3094. [CrossRef] [PubMed]
    [Google Scholar]
  41. Zakrzewska-Czerwińska J. , Mordarski M. , Goodfellow M. . ( 1988; ). DNA base composition and homology values in the classification of some Rhodococcus species. . J Gen Microbiol 134:, 2807–2813.[PubMed]
    [Google Scholar]
  42. Zhao B. , Wang H. , Mao X. , Li R. . ( 2009; ). Biodegradation of phenanthrene by a halophilic bacterial consortium under aerobic conditions. . Curr Microbiol 58:, 205–210. [CrossRef] [PubMed]
    [Google Scholar]
  43. Zhao B. , Wang H. , Li R. , Mao X. . ( 2010; ). Thalassospira xianhensis sp. nov., a polycyclic aromatic hydrocarbon-degrading marine bacterium. . Int J Syst Evol Microbiol 60:, 1125–1129. [CrossRef] [PubMed]
    [Google Scholar]
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