1887

Abstract

Four strains isolated in the last 15 years were revealed to be identical in their 16S rRNA gene sequences to MCRO19, the sequence of which was deposited in GenBank in 1995. In a polyphasic analysis including phenotypic and genotypic features, the five strains (including MCRO19), which had been isolated in four European countries, turned out to represent a unique taxonomic entity. They are scotochromogenic slow growers and are genetically related to the group that included and 15 other species. The novel species sp. nov. is proposed to accommodate these five strains. Strain FI-95228 ( = DSM 45397  = CCUG 58464) was chosen as the type strain. In addition, a thorough revision of the phenotypic and genotypic characters of the species related to was conducted which leads us to suggest the denomination of the ‘ complex’ for this group.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.025601-0
2011-07-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/7/1606.html?itemId=/content/journal/ijsem/10.1099/ijs.0.025601-0&mimeType=html&fmt=ahah

References

  1. Adékambi T. , Colson P. , Drancourt M. . ( 2003; ). rpoB-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteria. . J Clin Microbiol 41:, 5699–5708. [CrossRef].[PubMed].
    [Google Scholar]
  2. Altschul S. F. , Madden T. L. , Schäffer A. A. , Zhang J. , Zhang Z. , Miller W. , Lipman D. J. . ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res 25:, 3389–3402. [CrossRef].[PubMed].
    [Google Scholar]
  3. Böttger E. C. , Hirschel B. , Coyle M. B. . ( 1993; ). Mycobacterium genavense sp. nov.. Int J Syst Bacteriol 43:, 841–843. [CrossRef].[PubMed].
    [Google Scholar]
  4. CDC ( 1996; ). Standardized method for HPLC identification of mycobacteria. Atlanta:: Centers for Disease Prevention and Control, US Department of Health and Human Services, Public Health Service;.
    [Google Scholar]
  5. Cingolani A. , Sanguinetti M. , Antinori A. , Larocca L. M. , Ardito F. , Posteraro B. , Federico G. , Fadda G. , Ortona L. . ( 2000; ). Brief report: disseminated mycobacteriosis caused by drug-resistant Mycobacterium triplex in a human immunodeficiency virus-infected patient during highly active antiretroviral therapy. . Clin Infect Dis 31:, 177–179. [CrossRef].[PubMed].
    [Google Scholar]
  6. Devulder G. , Pérouse de Montclos M. , Flandrois J. P. . ( 2005; ). A multigene approach to phylogenetic analysis using the genus Mycobacterium as a model. . Int J Syst Evol Microbiol 55:, 293–302. [CrossRef].[PubMed].
    [Google Scholar]
  7. Fanti F. , Tortoli E. , Hall L. , Roberts G. D. , Kroppenstedt R. M. , Dodi I. , Conti S. , Polonelli L. , Chezzi C. . ( 2004; ). Mycobacterium parmense sp. nov.. Int J Syst Evol Microbiol 54:, 1123–1127. [CrossRef].[PubMed].
    [Google Scholar]
  8. Floyd M. M. , Guthertz L. S. , Silcox V. A. , Duffey P. S. , Jang Y. , Desmond E. P. , Crawford J. T. , Butler W. R. . ( 1996; ). Characterization of an SAV organism and proposal of Mycobacterium triplex sp. nov.. J Clin Microbiol 34:, 2963–2967.[PubMed].
    [Google Scholar]
  9. Floyd M. M. , Gross W. M. , Bonato D. A. , Silcox V. A. , Smithwick R. W. , Metchock B. , Crawford J. T. , Butler W. R. . ( 2000; ). Mycobacterium kubicae sp. nov., a slowly growing, scotochromogenic Mycobacterium . . Int J Syst Evol Microbiol 50:, 1811–1816.[PubMed].
    [Google Scholar]
  10. Haas W. H. , Butler W. R. , Kirschner P. , Plikaytis B. B. , Coyle M. B. , Amthor B. , Steigerwalt A. G. , Brenner D. J. , Salfinger M. et al. ( 1997; ). A new agent of mycobacterial lymphadenitis in children: Mycobacterium heidelbergense sp. nov.. J Clin Microbiol 35:, 3203–3209.[PubMed].
    [Google Scholar]
  11. Karassova V. , Weissfeiler J. , Krasznay E. . ( 1965; ). Occurrence of atypical mycobacteria in Macacus rhesus . . Acta Microbiol Acad Sci Hung 12:, 275–282.[PubMed].
    [Google Scholar]
  12. Kent P. T. , Kubica G. P. . ( 1985; ). Public Health Mycobacteriology. A Guide for the Level III Laboratory. Atlanta:: US Department of Health and Human Services;.
    [Google Scholar]
  13. Kirschner P. , Meier A. , Böttger E. C. . ( 1993a; ). Genotypic identification and detection of mycobacteria – facing novel and uncultured pathogens. . In Diagnostic Molecular Microbiology: Principles and Applications, pp. 173–190. Edited by Persing D. H. , Smith T. F. , Tenover F. C. , White T. J. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  14. Kirschner P. , Springer B. , Vogel U. , Meier A. , Wrede A. , Kiekenbeck M. , Bange F. C. , Böttger E. C. . ( 1993b; ). Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory. . J Clin Microbiol 31:, 2882–2889.[PubMed].
    [Google Scholar]
  15. Levi M. H. , Bartell J. , Gandolfo L. , Smole S. C. , Costa S. F. , Weiss L. M. , Johnson L. K. , Osterhout G. , Herbst L. H. . ( 2003; ). Characterization of Mycobacterium montefiorense sp. nov., a novel pathogenic Mycobacterium from moray eels that is related to Mycobacterium triplex . . J Clin Microbiol 41:, 2147–2152. [CrossRef].[PubMed].
    [Google Scholar]
  16. McNabb A. , Eisler D. , Adie K. , Amos M. , Rodrigues M. , Stephens G. , Black W. A. , Isaac-Renton J. . ( 2004; ). Assessment of partial sequencing of the 65-kilodalton heat shock protein gene (hsp65) for routine identification of Mycobacterium species isolated from clinical sources. . J Clin Microbiol 42:, 3000–3011. [CrossRef].[PubMed].
    [Google Scholar]
  17. Meier A. , Kirschner P. , Schröder K. H. , Wolters J. , Kroppenstedt R. M. , Böttger E. C. . ( 1993; ). Mycobacterium intermedium sp. nov.. Int J Syst Bacteriol 43:, 204–209. [CrossRef].[PubMed].
    [Google Scholar]
  18. Minnikin D. E. , Alshamaony L. , Goodfellow M. . ( 1975; ). Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. . J Gen Microbiol 88:, 200–204.[PubMed].[CrossRef]
    [Google Scholar]
  19. Pourahmad F. , Cervellione F. , Thompson K. D. , Taggart J. B. , Adams A. , Richards R. H. . ( 2008; ). Mycobacterium stomatepiae sp. nov., a slowly growing, non-chromogenic species isolated from fish. . Int J Syst Evol Microbiol 58:, 2821–2827. [CrossRef].[PubMed].
    [Google Scholar]
  20. Rastogi N. , Goh K. S. , Guillou N. , Labrousse V. . ( 1992; ). Spectrum of drugs against atypical mycobacteria: how valid is the current practice of drug susceptibility testing and the choice of drugs?. Zentralbl Bakteriol 277:, 474–484.[PubMed].[CrossRef]
    [Google Scholar]
  21. Rogall T. , Wolters J. , Flohr T. , Böttger E. C. . ( 1990; ). Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium . . Int J Syst Bacteriol 40:, 323–330. [CrossRef].[PubMed].
    [Google Scholar]
  22. Roth A. , Fischer M. , Hamid M. E. , Michalke S. , Ludwig W. , Mauch H. . ( 1998; ). Differentiation of phylogenetically related slowly growing mycobacteria based on 16S-23S rRNA gene internal transcribed spacer sequences. . J Clin Microbiol 36:, 139–147.[PubMed].
    [Google Scholar]
  23. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed].
    [Google Scholar]
  24. Selvarangan R. , Wu W. K. , Nguyen T. T. , Carlson L. D. , Wallis C. K. , Stiglich S. K. , Chen Y. C. , Jost K. C. Jr , Prentice J. L. et al. ( 2004; ). Characterization of a novel group of mycobacteria and proposal of Mycobacterium sherrisii sp. nov.. J Clin Microbiol 42:, 52–59. [CrossRef].[PubMed].
    [Google Scholar]
  25. Springer B. , Kirschner P. , Rost-Meyer G. , Schröder K. H. , Kroppenstedt R. M. , Böttger E. C. . ( 1993; ). Mycobacterium interjectum, a new species isolated from a patient with chronic lymphadenitis. . J Clin Microbiol 31:, 3083–3089.[PubMed].
    [Google Scholar]
  26. Springer B. , Stockman L. , Teschner K. , Roberts G. D. , Böttger E. C. . ( 1996; a). Two-laboratory collaborative study on identification of mycobacteria: molecular versus phenotypic methods. . J Clin Microbiol 34:, 296–303.[PubMed].
    [Google Scholar]
  27. Springer B. , Wu W. K. , Bodmer T. , Haase G. , Pfyffer G. E. , Kroppenstedt R. M. , Schröder K. H. , Emler S. , Kilburn J. O. et al. ( 1996; b). Isolation and characterization of a unique group of slowly growing mycobacteria: description of Mycobacterium lentiflavum sp. nov.. J Clin Microbiol 34:, 1100–1107.[PubMed].
    [Google Scholar]
  28. Stackebrandt E. , Frederiksen W. , Garrity G. M. , Grimont P. A. D. , Kämpfer P. , Maiden M. C. J. , Nesme X. , Rosselló-Mora R. , Swings J. et al. ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. . Int J Syst Evol Microbiol 52:, 1043–1047. [CrossRef].[PubMed].
    [Google Scholar]
  29. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef].[PubMed].
    [Google Scholar]
  30. Torkko P. , Suomalainen S. , Iivanainen E. , Tortoli E. , Suutari M. , Seppänen J. , Paulin L. , Katila M. L. . ( 2002; ). Mycobacterium palustre sp. nov., a potentially pathogenic, slowly growing mycobacterium isolated from clinical and veterinary specimens and from Finnish stream waters. . Int J Syst Evol Microbiol 52:, 1519–1525. [CrossRef].[PubMed].
    [Google Scholar]
  31. Tortoli E. . ( 2003; ). Impact of genotypic studies on mycobacterial taxonomy: the new mycobacteria of the 1990s. . Clin Microbiol Rev 16:, 319–354. [CrossRef].[PubMed].
    [Google Scholar]
  32. Tortoli E. . ( 2006; ). The new mycobacteria: an update. . FEMS Immunol Med Microbiol 48:, 159–178. [CrossRef].[PubMed].
    [Google Scholar]
  33. Tortoli E. , Bartoloni A. , Burrini C. , Colombrita D. , Mantella A. , Pinsi G. , Simonetti M. T. , Swierczynski G. , Böttger E. C. . ( 1996a; ). Characterization of an isolate of the newly described species Mycobacterium interjectum . . Zentralbl Bakteriol 283:, 286–294.[PubMed].[CrossRef]
    [Google Scholar]
  34. Tortoli E. , Kirschner P. , Bartoloni A. , Burrini C. , Manfrin V. , Mantella A. , Scagnelli M. , Scarparo C. , Simonetti M. T. , Böttger E. C. . ( 1996b; ). Isolation of an unusual mycobacterium from an AIDS patient. . J Clin Microbiol 34:, 2316–2319.[PubMed].
    [Google Scholar]
  35. Tortoli E. , Piersimoni C. , Kirschner P. , Bartoloni A. , Burrini C. , Lacchini C. , Mantella A. , Muzzi G. , Tosi C. P. et al. ( 1997; ). Characterization of mycobacterial isolates phylogenetically related to, but different from Mycobacterium simiae . . J Clin Microbiol 35:, 697–702.[PubMed].
    [Google Scholar]
  36. Tortoli E. , Bartoloni A. , Erba M. L. , Levrè E. , Lombardi N. , Mantella A. , Mecocci L. . ( 2002; ). Human infections due to Mycobacterium lentiflavum . . J Clin Microbiol 40:, 728–729. [CrossRef].[PubMed].
    [Google Scholar]
  37. Tortoli E. , Rindi L. , Goh K. S. , Katila M. L. , Mariottini A. , Mattei R. , Mazzarelli G. , Suomalainen S. , Torkko P. , Rastogi N. . ( 2005a; ). Mycobacterium florentinum sp. nov., isolated from humans. . Int J Syst Evol Microbiol 55:, 1101–1106. [CrossRef].[PubMed].
    [Google Scholar]
  38. Tortoli E. , Chianura L. , Fabbro L. , Mariottini A. , Martín-Casabona N. , Mazzarelli G. , Russo C. , Spinelli M. . ( 2005b; ). Infections due to the newly described species Mycobacterium parascrofulaceum . . J Clin Microbiol 43:, 4286–4287. [CrossRef].[PubMed].
    [Google Scholar]
  39. Turenne C. Y. , Thibert L. , Williams K. , Burdz T. V. , Cook V. J. , Wolfe J. N. , Cockcroft D. W. , Kabani A. . ( 2004a; ). Mycobacterium saskatchewanense sp. nov., a novel slowly growing scotochromogenic species from human clinical isolates related to Mycobacterium interjectum and Accuprobe-positive for Mycobacterium avium complex. . Int J Syst Evol Microbiol 54:, 659–667. [CrossRef].[PubMed].
    [Google Scholar]
  40. Turenne C. Y. , Cook V. J. , Burdz T. V. , Pauls R. J. , Thibert L. , Wolfe J. N. , Kabani A. . ( 2004b; ). Mycobacterium parascrofulaceum sp. nov., novel slowly growing, scotochromogenic clinical isolates related to Mycobacterium simiae . . Int J Syst Evol Microbiol 54:, 1543–1551. [CrossRef].[PubMed].
    [Google Scholar]
  41. van Ingen J. , Tortoli E. , Selvarangan R. , Coyle M. B. , Crump J. A. , Morrissey A. B. , Dekhuijzen P. N. , Boeree M. J. , van Soolingen D. . ( 2011; ). Mycobacterium sherrisii sp. nov., a slow-growing non-chromogenic species. . Int J Syst Evol Microbiol 61:, 1293–1298.[PubMed].[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.025601-0
Loading
/content/journal/ijsem/10.1099/ijs.0.025601-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error