1887

Abstract

During the course of a study on the bacterial diversity in Western Ghats, India, an actinobacterial strain, designated PC IW02, was isolated and characterized by a polyphasic taxonomic approach. Strain PC IW02 was a non-motile, Gram-positive, short rod that formed creamish white to yellow coloured colonies. 16S rRNA gene sequence analysis showed that the novel strain showed highest sequence similarity with type strains of members of the genus : (96.6 %), (96.5 %), (96.4 %) and (95.9 %). The phylogenetic tree suggested that strain PC IW02 could represent a member of a new genus of the family with the genus as closest clade. Pairwise sequence alignment with HKI 0089 and DSM 20547 showed similarities of 94.2 and 93.7 %, respectively. Strain PC IW02 had MK-8(H) as the major menaquinone. The major fatty acids were iso-C (43.4 %), iso-C H (17.2 %) and anteiso-C (9.9 %). The diagnostic cell-wall amino acid at position 3 of the peptide subunit was lysine; the interpeptide bridge consisted of Gly–Ser–Asp. The polar lipids present were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and phosphatidylserine, along with two unknown phospholipids. The genomic DNA G+C content of the isolate was 77 mol%. On the basis of phenotypic characteristics, including chemotaxonomic data, and 16S rRNA gene sequence similarities, strain PC IW02 represents a novel species in a new genus of the family for which the name gen. nov., sp. nov. is proposed. The type strain of is PC IW02 ( = MTCC 8338 = DSM 22967 = JCM 16038). An emended description of the family is provided.

Funding
This study was supported by the:
  • , CSIR , (Award NWP 006)
  • , DBT
  • , DBT
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2011-10-01
2020-10-28
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