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Abstract

A Gram-stain-negative, rod-shaped, non-spore-forming bacterium originating from a human clinical specimen was studied for its taxonomic position. 16S rRNA gene sequence similarity studies clearly allocated this strain (CCUG 58010) to the class , closely related to members of the genera and . was shown to be the most closely related species on the basis of 16S rRNA gene sequence similarity (97.5 %), followed by (96.8 %) and (96.4 %). Similarities to all other species of the genera and were in the range 93.9–96.2 %. Chemotaxonomic data (major ubiquinone: Q-8; major polar lipids: phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; and major fatty acids: summed feature 3 (Cω7 and/or iso-C 2-OH), C, Cω7 and C, with C 3-OH as hydroxylated fatty acid) supported the affiliation of the isolate to these genera, which share these chemotaxonomic traits. DNA–DNA hybridization of strain CCUG 58010 with the type strain of CCUG 35299 resulted in a relatedness value of 39.2 % (reciprocal, 50 %) and physiological and biochemical tests also allowed phenotypic differentiation of the isolate from the most closely related species. There is currently no justification for a division of the genera and and for this reason a proposal is made to transfer all species of the genus to the genus , as comb. nov., comb. nov., comb. nov. and comb. nov. Strain CCUG 58010 represents a novel species, for which the name sp. nov. is proposed, with the type strain CCUG 58010 ( = CCM 7792).

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2011-07-01
2019-09-22
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vol. , part 7, pp. 1528 - 1533

Phylogenetic analysis based on 16S rRNA gene sequences available from the European Molecular Biology Laboratory database (accession numbers are given in parentheses). The phylogenetic tree was reconstructed using the arbsoftware package (version December 2007; Ludwig , 2004), after multiple alignment of data with the arbalignment tool and the silvaSSURef 100 database (release August 2009; Pruesse 2007). Clustering with the neighbour-joining method was performed by using bootstrap values based on 1000 replications. Bar, 0.10 substitutions per nucleotide position.



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