1887

Abstract

Two Gram-positive, motile cocci, strains TF-9 and TF-16, were isolated from sea water of a tidal flat in Korea. Phylogenetic analysis based on 16S rDNA sequences showed that the strains fall within the radiation of the cluster comprising and , and this cluster joined the clade comprising species and and with a high bootstrap resampling value. Strains TF-9 and TF-16 contained MK-8, MK-7 and MK-6 as the predominant menaquinones. The major fatty acid was anteiso-C. The DNA G+C contents of strains TF-9 and TF-16 were respectively 48 and 49 mol%. The level of 16S rDNA identity between strains TF-9 and TF-16 was 98·9 %. Strain TF-9 and TF-16 respectively exhibited levels of 16S rDNA identity of 97·2–98·4 and 96·9–99·5 % to the type strains of , and . The mean level of DNA–DNA relatedness between strains TF-9 and TF-16 was 36·7 %. Strains TF-9 and TF-16 respectively exhibited levels of DNA–DNA relatedness of 7·1–43·4 and 3·5–80·9 % to DSM 20549, DSM 20747 and DSM 14505. On the basis of the phenotypic, phylogenetic and genomic data, strain TF-9 (=KCCM 41587=JCM 11543) should be placed as a novel species of the genus , for which the name sp. nov. is proposed, and strain TF-16 should be classified as a member of .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02557-0
2003-11-01
2021-03-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/53/6/ijs532013.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02557-0&mimeType=html&fmt=ahah

References

  1. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  2. Engelhardt M. A., Daly K., Swannell R. P. J., Head I. M. 2001; Isolation and characterization of a novel hydrocarbon-degrading, Gram-positive bacterium, isolated from intertidal beach sediment, and description of Planococcus alkanoclasticus sp. nov. J Appl Microbiol 90:237–247 [CrossRef]
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  4. Hao M. V., Komagata K. 1985; A new species of Planococcus , P . kocurii isolated from fish, frozen foods, and fish curing brine. J Gen Appl Microbiol 31:441–455 [CrossRef]
    [Google Scholar]
  5. Junge K., Gosink J. J., Hoppe H.-G., Staley J. T. 1998; Arthrobacter , Brachybacterium and Planococcus isolates identified from antarctic sea ice brine. Description of Planococcus mcmeekinii sp. nov. Syst Appl Microbiol 21306–314 [CrossRef]
    [Google Scholar]
  6. Kocur M., Páčová Z., Hodgkiss W., Martinec T. 1970; The taxonomic status of the genus Planococcus Migula 1894. Int J Syst Bacteriol 20:241–248 [CrossRef]
    [Google Scholar]
  7. Komagata K., Suzuki K. 1987; Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–203
    [Google Scholar]
  8. Lanyi B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  9. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  10. Levring T. 1946; Some culture experiments with Ulva and artificial seawater. Kungl Fysiografiska Sällsk Lund Förhandlingar 16:45–56
    [Google Scholar]
  11. Migula W. 1894; Über ein neues system der bakterien. Arb Bakteriol Inst Karlsruhe 1:235–238
    [Google Scholar]
  12. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [CrossRef]
    [Google Scholar]
  13. Nakagawa Y., Sakane T., Yokota A. 1996; Emendation of the genus Planococcus and transfer of Flavobacterium okeanokoites Zobell and Upham 1944 to the genus Planococcus as Planococcus okeanokoites comb. nov. Int J Syst Bacteriol 46:866–870 [CrossRef]
    [Google Scholar]
  14. Reddy G. S. N., Prakash J. S. S., Vairamani M., Prabhakar S., Matsumoto G. I., Shivaji S. 2002; Planococcus antarcticus and Planococcus psychrophilus spp. nov. isolated from cyanobacterial mat samples collected from ponds in Antarctica. Extremophiles 6:253–261 [CrossRef]
    [Google Scholar]
  15. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  16. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  17. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  18. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  19. Yoon J.-H., Lee S. T., Kim S.-B., Goodfellow M., Park Y.-H. 1997; Inter- and intraspecific genetic analysis of the genus Saccharomonospora with 16S to 23S ribosomal DNA (rDNA) and 23S to 5S rDNA internally transcribed spacer sequences. Int J Syst Bacteriol 47:661–669 [CrossRef]
    [Google Scholar]
  20. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [CrossRef]
    [Google Scholar]
  21. Yoon J.-H., Kang S.-S., Lee K.-C., Lee E. S., Kho Y. H., Kang K. H., Park Y.-H. 2001; Planomicrobium koreense gen. nov., sp. nov., a bacterium isolated from the Korean traditional fermented seafood jeotgal, and transfer of Planococcus okeanokoites (Nakagawa et al , 1996) and Planococcus mcmeekinii (Junge et al . 1998) to the genus Planomicrobium . Int J Syst Evol Microbiol 511511–1520
    [Google Scholar]
  22. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H. 2003; Microbulbifer salipaludis sp. nov. a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53:53–57 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02557-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02557-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error