1887

Abstract

Two Gram-positive, motile cocci, strains TF-9 and TF-16, were isolated from sea water of a tidal flat in Korea. Phylogenetic analysis based on 16S rDNA sequences showed that the strains fall within the radiation of the cluster comprising and , and this cluster joined the clade comprising species and and with a high bootstrap resampling value. Strains TF-9 and TF-16 contained MK-8, MK-7 and MK-6 as the predominant menaquinones. The major fatty acid was anteiso-C. The DNA G+C contents of strains TF-9 and TF-16 were respectively 48 and 49 mol%. The level of 16S rDNA identity between strains TF-9 and TF-16 was 98·9 %. Strain TF-9 and TF-16 respectively exhibited levels of 16S rDNA identity of 97·2–98·4 and 96·9–99·5 % to the type strains of , and . The mean level of DNA–DNA relatedness between strains TF-9 and TF-16 was 36·7 %. Strains TF-9 and TF-16 respectively exhibited levels of DNA–DNA relatedness of 7·1–43·4 and 3·5–80·9 % to DSM 20549, DSM 20747 and DSM 14505. On the basis of the phenotypic, phylogenetic and genomic data, strain TF-9 (=KCCM 41587=JCM 11543) should be placed as a novel species of the genus , for which the name sp. nov. is proposed, and strain TF-16 should be classified as a member of .

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2003-11-01
2020-01-28
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References

  1. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  2. Engelhardt, M. A., Daly, K., Swannell, R. P. J. & Head, I. M. ( 2001; ). Isolation and characterization of a novel hydrocarbon-degrading, Gram-positive bacterium, isolated from intertidal beach sediment, and description of Planococcus alkanoclasticus sp. nov. J Appl Microbiol 90, 237–247.[CrossRef]
    [Google Scholar]
  3. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  4. Hao, M. V. & Komagata, K. ( 1985; ). A new species of Planococcus, P. kocurii isolated from fish, frozen foods, and fish curing brine. J Gen Appl Microbiol 31, 441–455.[CrossRef]
    [Google Scholar]
  5. Junge, K., Gosink, J. J., Hoppe, H.-G. & Staley, J. T. ( 1998; ). Arthrobacter, Brachybacterium and Planococcus isolates identified from antarctic sea ice brine. Description of Planococcus mcmeekinii, sp. nov. Syst Appl Microbiol 21, 306–314.[CrossRef]
    [Google Scholar]
  6. Kocur, M., Páčová, Z., Hodgkiss, W. & Martinec, T. ( 1970; ). The taxonomic status of the genus Planococcus Migula 1894 . Int J Syst Bacteriol 20, 241–248.[CrossRef]
    [Google Scholar]
  7. Komagata, K. & Suzuki, K. ( 1987; ). Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–203.
    [Google Scholar]
  8. Lanyi, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  9. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
    [Google Scholar]
  10. Levring, T. ( 1946; ). Some culture experiments with Ulva and artificial seawater. Kungl Fysiografiska Sällsk Lund Förhandlingar 16, 45–56.
    [Google Scholar]
  11. Migula, W. ( 1894; ). Über ein neues system der bakterien. Arb Bakteriol Inst Karlsruhe 1, 235–238.
    [Google Scholar]
  12. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  13. Nakagawa, Y., Sakane, T. & Yokota, A. ( 1996; ). Emendation of the genus Planococcus and transfer of Flavobacterium okeanokoites Zobell and Upham 1944 to the genus Planococcus as Planococcus okeanokoites comb. nov. Int J Syst Bacteriol 46, 866–870.[CrossRef]
    [Google Scholar]
  14. Reddy, G. S. N., Prakash, J. S. S., Vairamani, M., Prabhakar, S., Matsumoto, G. I. & Shivaji, S. ( 2002; ). Planococcus antarcticus and Planococcus psychrophilus spp. nov. isolated from cyanobacterial mat samples collected from ponds in Antarctica. Extremophiles 6, 253–261.[CrossRef]
    [Google Scholar]
  15. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  16. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  17. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  18. Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H. ( 1996; ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46, 502–505.[CrossRef]
    [Google Scholar]
  19. Yoon, J.-H., Lee, S. T., Kim, S.-B., Goodfellow, M. & Park, Y.-H. ( 1997; ). Inter- and intraspecific genetic analysis of the genus Saccharomonospora with 16S to 23S ribosomal DNA (rDNA) and 23S to 5S rDNA internally transcribed spacer sequences. Int J Syst Bacteriol 47, 661–669.[CrossRef]
    [Google Scholar]
  20. Yoon, J.-H., Lee, S. T. & Park, Y.-H. ( 1998; ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48, 187–194.[CrossRef]
    [Google Scholar]
  21. Yoon, J.-H., Kang, S.-S., Lee, K.-C., Lee, E. S., Kho, Y. H., Kang, K. H. & Park, Y.-H. ( 2001; ). Planomicrobium koreense gen. nov., sp. nov., a bacterium isolated from the Korean traditional fermented seafood jeotgal, and transfer of Planococcus okeanokoites ( Nakagawa et al. 1996 ) and Planococcus mcmeekinii ( Junge et al. 1998 ) to the genus Planomicrobium. Int J Syst Evol Microbiol 51, 1511–1520.
    [Google Scholar]
  22. Yoon, J.-H., Kim, I.-G., Shin, D.-Y., Kang, K. H. & Park, Y.-H. ( 2003; ). Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53, 53–57.[CrossRef]
    [Google Scholar]
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