1887

Abstract

Two Gram-negative, aerobic, non-pigmented, non-motile, rod-shaped bacteria, strains KMM 9015 and KMM 9018, were isolated from a sample of shallow sediment collected from the Sea of Japan. An analysis of the nearly complete 16S rRNA gene sequences showed that the isolates were very close to each other phylogenetically (99.9 % sequence similarity) and their close relatives were FF3 (95.8 and 95.9 %, respectively) and members of the genera (95.7–95.1 and 95.8–95.2 %), (95.0–94.2 and 95.1–94.2 %) and (96.3–94.8 and 96.2–94.7 %) of the class . In phylogenetic trees based on 16S rRNA gene sequences, strains KMM 9015 and KMM 9018 were positioned as a distinct phylogenetic line adjacent to . The major isoprenoid quinone was Q-10, the polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unknown lipid and the major fatty acid was Cω7, followed by 11-methyl Cω7, in both strains. The DNA G+C contents of strains KMM 9015 and KMM 9018 were 60.2 and 61.9 mol%, respectively. Based on distinctive phenotypic characteristics and phylogenetic analysis, strains KMM 9015 and KMM 9018 represent a novel species in a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is strain KMM 9018 ( = NRIC 0784  = JCM 16874).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.025551-0
2011-07-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/7/1572.html?itemId=/content/journal/ijsem/10.1099/ijs.0.025551-0&mimeType=html&fmt=ahah

References

  1. Arahal D. R. , Macián M. C. , Garay E. , Pujalte M. J. . ( 2005; ). Thalassobius mediterraneus gen. nov., sp. nov., and reclassification of Ruegeria gelatinovorans as Thalassobius gelatinovorus comb. nov.. Int J Syst Evol Microbiol 55:, 2371–2376. [CrossRef].[PubMed].
    [Google Scholar]
  2. Bruns A. , Rohde M. , Berthe-Corti L. . ( 2001; ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51:, 1997–2006.[PubMed].[CrossRef]
    [Google Scholar]
  3. Buchan A. , González J. M. , Moran M. A. . ( 2005; ). Overview of the marine Roseobacter lineage. . Appl Environ Microbiol 71:, 5665–5677. [CrossRef].[PubMed].
    [Google Scholar]
  4. Collins M. D. , Shah H. N. . ( 1984; ). Fatty acid, menaquinone and polar lipid composition of Rothia dentosacariosa . . Arch Microbiol 137:, 247–249. [CrossRef].
    [Google Scholar]
  5. Ezaki T. , Hashimoto Y. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef].
    [Google Scholar]
  6. Folch J. , Lees M. , Sloane Stanley G. H. . ( 1957; ). A simple method for the isolation and purification of total lipids from animal tissues. . J Biol Chem 226:, 497–509.[PubMed].
    [Google Scholar]
  7. Garrity G. M. , Bell J. A. , Lilburn T. . ( 2005; ). Order III. Rhodobacterales ord. nov.. In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2C, p.161. Edited by Brenner D. J. , Krieg N. R. , Staley J. T. , Garrity G. M. . . New York:. Springer;.
    [Google Scholar]
  8. Garrity G. M. , Bell J. A. , Lilburn T. . ( 2006; ). Rhodobacterales ord. nov. In List of New Names and New Combinations Previously Effectively, but not Validly, Published, Validation List no. 107. . Int J Syst Evol Microbiol 56:, 1–6.[PubMed].[CrossRef]
    [Google Scholar]
  9. Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  10. Labrenz M. , Collins M. D. , Lawson P. A. , Tindall B. J. , Schumann P. , Hirsch P. . ( 1999; ). Roseovarius tolerans gen. nov., sp. nov., a budding bacterium with variable bacteriochlorophyll a production from hypersaline Ekho Lake. . Int J Syst Bacteriol 49:, 137–147. [CrossRef].[PubMed].
    [Google Scholar]
  11. Lafay B. , Ruimy R. , Rausch De Traubenberg C. , Breittmayer V. , Gauthier M. J. , Christen R. . ( 1995; ). Roseobacter algicola sp. nov., a new marine bacterium isolated from the phycosphere of the toxin-producing dinoflagellate Prorocentrum lima . . Int J Syst Bacteriol 45:, 290–296. [CrossRef].[PubMed].
    [Google Scholar]
  12. Leifson E. . ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85:, 1183–1184.[PubMed].
    [Google Scholar]
  13. Marmur J. , Doty P. . ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5:, 109–118. [CrossRef].[PubMed].
    [Google Scholar]
  14. Martens T. , Heidorn T. , Pukall R. , Simon M. , Tindall B. J. , Brinkhoff T. . ( 2006; ). Reclassification of Roseobacter gallaeciensis Ruiz-Ponte et al. 1998 as Phaeobacter gallaeciensis gen. nov., comb. nov., description of Phaeobacter inhibens sp. nov., reclassification of Ruegeria algicola (Lafay et al. 1995) Uchino et al. 1999 as Marinovum algicola gen. nov., comb. nov., and emended descriptions of the genera Roseobacter, Ruegeria and Leisingera . . Int J Syst Evol Microbiol 56:, 1293–1304. [CrossRef].[PubMed].
    [Google Scholar]
  15. Owen R. J. , Hill L. R. , Lapage S. P. . ( 1969; ). Determination of DNA base compositions from melting profiles in dilute buffers. . Biopolymers 7:, 503–516. [CrossRef].[PubMed].
    [Google Scholar]
  16. Pearson W. R. , Lipman D. J. . ( 1988; ). Improved tools for biological sequence comparison. . Proc Natl Acad Sci U S A 85:, 2444–2448. [CrossRef].[PubMed].
    [Google Scholar]
  17. Romanenko L. A. , Schumann P. , Zhukova N. V. , Rohde M. , Mikhailov V. V. , Stackebrandt E. . ( 2003; ). Oceanisphaera litoralis gen. nov., sp. nov., a novel halophilic bacterium from marine bottom sediments. . Int J Syst Evol Microbiol 53:, 1885–1888. [CrossRef].[PubMed].
    [Google Scholar]
  18. Romanenko L. A. , Uchino M. , Falsen E. , Frolova G. M. , Zhukova N. V. , Mikhailov V. V. . ( 2005; ). Pseudomonas pachastrellae sp. nov., isolated from a marine sponge. . Int J Syst Evol Microbiol 55:, 919–924. [CrossRef].[PubMed].
    [Google Scholar]
  19. Rüger H.-J. , Höfle M. G. . ( 1992; ). Marine star-shaped-aggregate-forming bacteria: Agrobacterium atlanticum sp. nov.; Agrobacterium meteori sp. nov.; Agrobacterium ferrugineum sp. nov., nom. rev.; Agrobacterium gelatinovorum sp. nov., nom. rev.; and Agrobacterium stellulatum sp. nov., nom. rev.. Int J Syst Bacteriol 42:, 133–143. [CrossRef].[PubMed].
    [Google Scholar]
  20. Ruiz-Ponte C. , Cilia V. , Lambert C. , Nicolas J. L. . ( 1998; ). Roseobacter gallaeciensis sp. nov., a new marine bacterium isolated from rearings and collectors of the scallop Pecten maximus . . Int J Syst Bacteriol 48:, 537–542.[PubMed].[CrossRef]
    [Google Scholar]
  21. Sasser M. . , ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI, Inc;.
    [Google Scholar]
  22. Schaefer J. K. , Goodwin K. D. , McDonald I. R. , Murrell J. C. , Oremland R. S. . ( 2002; ). Leisingera methylohalidivorans gen. nov., sp. nov., a marine methylotroph that grows on methyl bromide. . Int J Syst Evol Microbiol 52:, 851–859. [CrossRef].[PubMed].
    [Google Scholar]
  23. Shida O. , Takagi H. , Kadowaki K. , Nakamura L. K. , Komagata K. . ( 1997; ). Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus . . Int J Syst Bacteriol 47:, 289–298. [CrossRef].[PubMed].
    [Google Scholar]
  24. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–655. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  25. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef].[PubMed].
    [Google Scholar]
  26. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef].[PubMed].
    [Google Scholar]
  27. Uchino Y. , Hirata A. , Yokota A. , Sugiyama J. . ( 1998; ). Reclassification of marine Agrobacterium species: proposals of Stappia stellulata gen. nov., comb. nov., Stappia aggregata sp. nov., nom. rev., Ruegeria atlantica gen. nov., comb. nov., Ruegeria gelatinovora comb. nov., Ruegeria algicola comb. nov., and Ahrensia kieliense gen. nov., sp. nov., nom. rev.. J Gen Appl Microbiol 44:, 201–210. [CrossRef]
    [Google Scholar]
  28. Uchino Y. , Hirata A. , Yokota A. , Sugiyama J. . ( 1999;). Ruegeria algicola comb. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB, List no. 68. . Int J Syst Bacteriol 49:, 1–3.[CrossRef]
    [Google Scholar]
  29. Vandecandelaere I. , Segaert E. , Mollica A. , Faimali M. , Vandamme P. . ( 2008; ). Leisingera aquimarina sp. nov., isolated from a marine electroactive biofilm, and emended descriptions of Leisingera methylohalidivorans Schaefer et al. 2002, Phaeobacter daeponensis Yoon et al. 2007 and Phaeobacter inhibens Martens et al. 2006. . Int J Syst Evol Microbiol 58:, 2788–2793. [CrossRef].[PubMed].
    [Google Scholar]
  30. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. et al. ( 1987; ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  31. Yoon J.-H. , Kang S.-J. , Lee S.-Y. , Oh T.-K. . ( 2007; ). Phaeobacter daeponensis sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. . Int J Syst Evol Microbiol 57:, 856–861. [CrossRef].[PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.025551-0
Loading
/content/journal/ijsem/10.1099/ijs.0.025551-0
Loading

Data & Media loading...

Supplements

Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences available from the GenBank/EMBL/DDBJ databases (accession numbers in parentheses) showing the relationship of strains KMM 9015 and KMM 9018 and related genera of the class . [PDF](41 KB)

PDF

Two-dimensional TLC of polar lipids of strain KMM 9018 . DPG, Diphosphatidylglycerol; PC, phosphatidylcholine, PE, phosphatidylethanolamine; PG, phosphatidylglycerol; L, unknown lipid.

IMAGE

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error