1887

Abstract

Two strains (JA317 and JA559) of spiral shaped, spheroplast-forming, anaerobic, Gram-negative, motile purple non-sulfur bacteria were isolated from rhizosphere soils of paddy and were characterized by a polyphasic taxonomic approach. Bacteriochlorophyll and carotenoids, rhodopin, lycopene and rhodopin glucoside, were present as photosynthetic pigments. Intracellular photosynthetic membranes were of stacked type. The major fatty acids were Cω7, C and Cω6/Cω7 in both strains. The genomic DNA G+C content was 63.3±0.8 mol%. The two strains were closely related (mean DNA–DNA hybridization >85 %). Phylogenetic analysis showed that the strains clustered with the species of the genus , which belongs to the family within the class . Based on 16S rRNA gene sequence analysis, strains JA317 and JA559 showed highest sequence similarity with the type strains of (98.2 %), (97.4 %) and (97.1 %) of the family . Strain JA317 can be clearly distinguished from with respect to spheroplast formation and several other morphological and physiological properties. DNA–DNA relatedness of strain JA317 with its closest relatives of the genus was less than 55 %. It is evident from the phenotypic, chemotaxonomic and molecular genetic evidence that strain JA317 represents a novel species of the genus , for which the name sp. nov., is proposed. The type strain of the species is JA317 ( = NBRC 104938  = KCTC 5704).

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2011-07-01
2019-10-24
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vol. , part 7, pp. 1656 - 1661

Phase-contrast (a, b) and fluorescent-stained (c, d) micrographs of strain JA317 and JA145 , respectively, depicting the presence of spherical bodies in the former and their absence in the latter.

(a) Electron micrograph of a negatively stained cell of strain JA317 showing a polar flagellum. (b) Electron micrograph of an ultrathin section of a cell of strain JA317 , showing photosynthetic membrane present as lamellar stacks.

(a). Whole-cell absorption spectra of strain JA317 . (b) Acetone absorption spectra of strain JA317 .

Phylogenetic tree based on almost complete 16S rRNA gene sequences showing the relationship of strains JA317 and JA559 within the class .

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