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Volume 61, Issue 11

sp. nov., a nitrate-reducing bacterium isolated from sludge of a leachate treatment plant Free

Abstract

A Gram-reaction-negative, non-spore-forming, rod-shaped, aerobic bacterial strain, designated MJ11, was isolated from sludge of a leachate treatment plant in Daejeon, South Korea, and was characterized taxonomically by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ11 belonged to the family , class , and was most closely related to Ca-34 (97.9 % sequence similarity) and CCUG 50899 (96.4 %). Comparative sequence analyses of the additional phylogenetic marker genes , and confirmed the affiliation of strain MJ11 to the genus . The G+C content of the genomic DNA of strain MJ11 was 59.3 mol%. The detection of a quinone system with ubiquinone Q-10 as the predominant respiratory lipoquinone, a fatty acid profile with Cω7 (62.6 %) and C cyclo ω8 (14.2 %) as the major components, a polar lipid profile with phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol and unknown aminolipids AL1 and AL2 as major polar lipids and spermidine and putrescine as the predominant polyamines also supported the affiliation of strain MJ11 to the genus . The DNA–DNA relatedness between strain MJ11 and DSM 22292 was 29±7 %, clearly showing that the isolate constitutes a new genospecies. Strain MJ11 could be clearly differentiated from its closest neighbours on the basis of its phenotypic, genotypic and chemotaxonomic features. Therefore, strain MJ11 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MJ11 ( = KCTC 22458  = JCM 16234).

  • Published Online:
Funding
This study was supported by the:
  • GAIA Project (Award 173-101-034)
  • Ministry of Environment, Republic of Korea
  • Korea Brain Pool Program of 2011 (Award 111S-4-5-0032)
© 2011 IUMS
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2011-11-01
2024-04-19
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Ochrobactrum daejeonense sp. nov., a nitrate-reducing bacterium isolated from sludge of a leachate treatment plant
Int J Syst Evol Microbiol 61, 2690 (2011); https://doi.org/10.1099/ijs.0.025510-0
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