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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 62, Issue 2

gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt mine Free

Abstract

A novel, moderately halophilic, rod-shaped bacterium, designated strain YIM D17, was isolated from a sample of sediment from a salt mine in Yunnan, south-western China. The taxonomy of strain YIM D17 was investigated using a polyphasic approach. Strain YIM D17 was Gram-stain-negative, strictly aerobic and non-motile and formed pink colonies on marine agar. Optimal growth occurred at 37 °C, pH 7.5–8.0 and in the presence of 10–15 % (w/v) NaCl. The major menaquinone was MK-7. The polar lipid profile was composed predominantly of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, one phospholipid, one glycolipid and one aminolipid. Minor amounts of other lipids were also detectable. The predominant cellular fatty acids were iso-Cω9/10-methyl-C (24.0 %), iso-C (23.6 %) and Cω7/Cω6 (13.8 %). The DNA G+C content was 43.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that the isolate formed a distinct clade with the genera and (both in the phylum ) and was related to the species , and , with sequence similarities of 85.6 %, 83.0 % and 82.8 % to the respective type strains. On the basis of its phenotypic, chemotaxonomic and phylogenetic features, strain YIM D17 represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain is YIM D17 ( = ACCC 10716 = DSM 21935).

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Funding
This study was supported by the:
  • Ministry of Environmental Protection of China
  • National Key Sciences and Technology Program for Water Solutions (Award 2008ZX07102-004)
  • National Natural Science Foundation of China (NSFC) (Award 31000003 and 30860013)
  • Ministry of Science and Technology of China (Award 2007AA021306)
  • Yunnan Provincial Sciences and Technology Department (Award 2009DA002, 2009CD012 and 2005PY01-1)
  • Yunnan University (Award 2009C14Q and 2008YB005)
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2012-02-01
2025-01-14
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Fodinibius salinus gen. nov., sp. nov., a moderately halophilic bacterium isolated from a salt mine
Int J Syst Evol Microbiol 62, 390 (2012); https://doi.org/10.1099/ijs.0.025502-0
/content/journal/ijsem/10.1099/ijs.0.025502-0
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