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Abstract

A novel non-sporulating, non-motile, catalase- and oxidase-positive, strictly aerobic, Gram-negative, rod-shaped bacterial strain, designated DCA-1, was isolated from activated sludge collected from a butachlor wastewater treatment facility. The strain was able to degrade about 85 % of 100 mg butachlor l within 5 days of incubation. Growth occurred in the presence of 0–6 % (w/v) NaCl [optimum, 1 % (w/v) NaCl] and at pH 5.5–9.0 (optimum, pH 7.0) and 15–35 °C (optimum, 25–30 °C). Vesicular internal membrane structures and photoheterotrophic growth were not observed. The major respiratory quinone was ubiquinone 10 (Q-10) and the major cellular fatty acids were Cω7 and 11-methyl Cω7. The genomic DNA G+C content of strain DCA-1 was 62.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparison revealed that strain DCA-1 was a member of the family and was related most closely to the type strain of (96.5 % sequence similarity). The combination of phylogenetic analysis, phenotypic characteristics and chemotaxonomic data supports the suggestion that strain DCA-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DCA-1 ( = CGMCC 1.7745  = DSM 21823). In addition, based on the characterization data obtained in this study, it is proposed that should be reclassified as comb. nov. (type strain JA139  = DSM 18774  = CCUG 53722  = JCM 14338). An emended description of the genus is also presented.

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2011-08-01
2020-01-24
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Transmission electron micrographs of a negatively stained cell of strain DCA-1 (A), which are characterized by a rod shape (0.6–0.7×1.8–4.5 µm) and lack of sporulation and flagella, and thin sections of cells of strain DCA-1 (B), in which vesicular internal membrane structures are not observed. Bars, 1.0 µm (A) and 0.5 µm (B).

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Time-course of degradation of butachlor by strain DCA-1 in minimal medium supplemented with 100 mg butachlor l . Filled squares and filled triangles indicate butachlor control and butachlor inoculated with strain DCA-1 , respectively.

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Phylogenetic trees showing the phylogenetic positions of strains DCA-1 and related taxa inferred from 16S rRNA gene sequences, reconstructed using maximum-likelihood and maximum-parsimony methods. [PDF](62 KB)

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