1887

Abstract

Seven isolates of a slowly growing, non-chromogenic species were obtained from sputum and bronchial lavage fluid samples from elderly patients in different regions of Japan. These isolates were distinguished from related non-tuberculous species by colony morphology, positive results for Tween hydrolysis, catalase at 68 °C, nitrate reductase and pyrazinamidase and negative results for semi-quantitative catalase, urease and arylsulfatase. The mycolic acid pattern obtained by HPLC revealed a single cluster of late-eluting mycolic acids similar to but different from those of ATCC 29571. The 16S rRNA gene, 16S–23S internal transcribed spacer (ITS), and sequences were unique in comparison with those of other mycobacteria. Comparison of 16S rRNA gene sequences showed that the isolates were most closely related to H37Rv (21 base differences in 1508 bp; 98.6 % 16S rRNA gene sequence similarity). A representative strain, GTC 2738, showed 91.9 % sequence similarity with strain M, 95 % sequence similarity with CIP 104589 and 81.1 % 16S–23S ITS sequence similarity with ATCC 14470. Phylogenetic analysis of concatenated sequences of the 16S rRNA, and genes showed that strain GTC 2738 was located on a distinct clade adjacent to , and , with bootstrap values of 81 %. DNA–DNA hybridization demonstrated less than 70 % reassociation with type strains of genetically related species and supported the novel species status of the isolates. On the basis of this evidence, a novel species with the name sp. nov. is proposed. The type strain, isolated from a sputum sample, is strain GTC 2738( = JCM 14233 = CCUG 53584).

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2011-08-01
2019-10-17
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