1887

Abstract

In addition to the grapevine flavescence dorée phytoplasmas, other members of taxonomic group 16SrV phytoplasmas infect grapevines, alders and species of the genera and in Europe. In order to investigate which phytoplasmas constitute discrete, species-level taxa, several strains were analysed by comparing their 16S rRNA gene sequences and a set of five housekeeping genes. Whereas 16S rRNA gene sequence similarity values were >97.5 %, the proposed threshold to distinguish two ‘ Phytoplasma’ taxa, phylogenetic analysis of the combined sequences of the , -, -, and - genetic loci showed that two discrete phylogenetic clusters could be clearly distinguished. The first cluster grouped flavescence dorée (FD) phytoplasmas, alder yellows (AldY) phytoplasmas, (CL) phytoplasmas and the Palatinate grapevine yellows (PGY) phytoplasmas. The second cluster comprised stunt (RS) phytoplasmas. In addition to the specificity of the insect vector, the stunt phytoplasma contained specific sequences in the 16S rRNA gene. Hence, the stunt phytoplasma 16S rRNA gene was sufficiently differentiated to represent a novel putative taxon: ‘ Phytoplasma rubi’.

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2011-09-01
2019-10-21
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vol. , part 9, pp. 2129 - 2134

Origin and sequence accession numbers of phytoplasma isolates used in this study.

Primers used for gene amplification and PCR conditions.

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