1887

Abstract

Flow cytometric sorting based on its large cell size enabled an enriched fraction of cells to be obtained from the rumen contents of a sheep. Phylogenetic analysis based on cloned 16S rDNA sequences indicated that the bacterium is a member of the low-G+C Gram-positive bacterial cluster. and were the most closely related known species, with sequence similarities of only 86·3–88·1 %. Fluorescently labelled 16S rRNA-targeted oligonucleotide probes specific for were designed and applied to the rumen sample from which the enriched fraction was obtained. The probes hybridized specifically with the large, morphologically conspicuous cells.

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2003-09-01
2019-10-21
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