1887

Abstract

A bacterial strain, designated an-8, was isolated from a freshwater shrimp culture pond in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain an-8 were Gram-reaction-negative, aerobic, rod-shaped and non-motile, formed yellow-pigmented colonies and grew at 15–30 °C (optimum 25 °C), pH 7–8 (optimum pH 8.0) and in 0–1 % (w/v) NaCl (optimum 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain an-8 belonged to the genus and its most closely related neighbours were DS-20 and R2A1-13 with sequence similarities of 95.1 and 94.9 %, respectively. Strain an-8 contained iso-C, summed feature 3 (Cω6 and/or Cω7), iso-C 3-OH, iso-C 3-OH, iso-C 3-OH and iso-C as the major fatty acids. The major isoprenoid quinone was MK-6. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidyldimethylethanolamine, phosphatidylserine and several unidentified polar lipids. The G+C content of the genomic DNA was 39.8 mol%. On the basis of the phylogenetic and phenotypic data, strain an-8 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is an-8 ( = BCRC 17965  = LMG 25203).

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2011-06-01
2019-12-06
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Two-dimensional thin-layer chromatogram of the polar lipids of an-8 (A), DS-20 (B) and R2A1-13 (C). Data for all strains from this study. PE, phosphatidylethanolamine; PDE, phosphatidyldimethylethanolamine; PS, phosphatidylserine; PL1-PL3, unknown phospholipids; AL1-AL4, unknown aminolipids. The TLC plates were sprayed with molybdatophosphoric acid for detection of total polar lipids.

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GN2 microplate oxidation data that can be used to distinguish strain an-8 from and . [PDF] (35kb)

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