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Abstract

An orange-pigmented, Gram-staining-negative, non-motile, filament-forming, rod-shaped bacterium (BUZ 3) was isolated from a coastal mud sample from the North Sea (Fedderwardersiel, Germany) and characterized taxonomically using a polyphasic approach. According to 16S rRNA gene sequence data, it belonged to the family exhibiting low 16S rRNA gene sequence similarity (<90 %) with members of the genera and . The DNA G+C content was 52.0 mol%. The major fatty acids were summed feature 3 (comprising Cω7 and/or iso-C 2-OH), Cω5 and iso-C 3-OH. The major polar lipids consisted of phosphatidylethanolamine and several aminolipids. On the basis of phenotypic, chemotaxonomic and phylogenetic data, it is proposed that strain BUZ 3 represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is BUZ 3 ( = DSM 22564  = CCUG 58137).

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2011-06-01
2020-01-19
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Absorption spectra of the ethanol extracted pigments of strain BUZ 3 and its closest phylogenetic neighbours DSM 19387 , DSM 74 and BUZ 2 . Data were normalized by dividing values by the highest value.

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Two-dimensional thin-layer chromatograph of the polar lipid extract obtained from strain BUZ 3 sprayed with 5 % molybdophosphoric acid in ethanol and heated. PE, Phosphatidylethanolamine; PL1, unidentified phospholipid; PN1–PN3, unidentified aminophospholipids; AL1–AL3, unidentified aminolipids; L1, L2, lipids.

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