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Abstract

Two Gram-negative, motile, non-spore-forming and moderately halophilic rods (strains SW-47 and SW-49) were isolated from sea water of the East Sea in Korea and subjected to a polyphasic taxonomic study. The two strains grew optimally between 30 and 37 °C, and grew at 4 and 44 °C but not at temperatures above 45 °C. They grew optimally in the presence of 2–5 % (w/v) NaCl, but did not grow in the absence of NaCl. Strains SW-47 and SW-49 had ubiquinone-8 (Q-8) as the predominant respiratory lipoquinone and C 7 and/or iso-C 2OH, C and C 7 as the major fatty acids, which are consistent with the corresponding data for . The DNA G+C contents of strains SW-47 and SW-49 were 45 and 44 mol%, respectively. Strains SW-47 and SW-49 showed a high level of 16S rDNA sequence similarity (99·9 %) and a mean level of DNA–DNA relatedness of 96·5 %. Phylogenetic analyses based on 16S rDNA sequences showed that the two strains form a coherent cluster with . Strains SW-47 and SW-49 exhibited levels of 16S rDNA sequence similarity of 99·3 and 99·1 %, respectively, with DSM 6062 and of less than 89·4 % with other species used in the phylogenetic analyses. CIP 105339 was found to be more closely related to the genus than to the genus . On the basis of phenotypic properties and phylogenetic and genomic data, strains SW-47 and SW-49 represent a new species of the genus , for which the name (type strain SW-47=KCCM 41638=JCM 11804) is proposed.

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2003-09-01
2020-01-24
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vol. , part 5, pp. 1625-1630

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