@article{mbs:/content/journal/ijsem/10.1099/ijs.0.02535-0, author = "Hoover, Richard B. and Pikuta, Elena V. and Bej, Asim K. and Marsic, Damien and Whitman, William B. and Tang, Jane and Krader, Paul", title = "Spirochaeta americana sp. nov., a new haloalkaliphilic, obligately anaerobic spirochaete isolated from soda Mono Lake in California", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2003", volume = "53", number = "3", pages = "815-821", doi = "https://doi.org/10.1099/ijs.0.02535-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.02535-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A novel, obligately anaerobic, mesophilic, haloalkaliphilic spirochaete, strain ASpG1T, was isolated from sediments of the alkaline, hypersaline Mono Lake in California, USA. Cells of the Gram-negative strain were motile and spirochaete-shaped with sizes of 0·2–0·22×8–18 μm. Growth of the strain was observed between 10 and 44 °C (optimum 37 °C), in 2–12 % (w/v) NaCl (optimum 3 % NaCl) and between pH 8 and 10·5 (optimum pH 9·5). The novel strain was strictly alkaliphilic, required high concentrations of carbonates in the medium and was capable of utilizing d-glucose, fructose, maltose, sucrose, starch and d-mannitol. End products of glucose fermentation were H2, acetate, ethanol and formate. Strain ASpG1T was resistant to kanamycin and rifampicin, but sensitive to gentamicin, tetracycline and chloramphenicol. The G+C content of its DNA was 58·5 mol%. DNA–DNA hybridization analysis of strain ASpG1T with its most closely related species, Spirochaeta alkalica Z-7491T, revealed a hybridization value of only 48·7 %. On the basis of its physiological and molecular properties, strain ASpG1T appears to represent a novel species of the genus Spirochaeta, for which the name Spirochaeta americana is proposed (type strain ASpG1T=ATCC BAA-392T=DSM 14872T).", }