1887

Abstract

A novel, obligately anaerobic, mesophilic, haloalkaliphilic spirochaete, strain ASpG1, was isolated from sediments of the alkaline, hypersaline Mono Lake in California, USA. Cells of the Gram-negative strain were motile and spirochaete-shaped with sizes of 0·2–0·22×8–18 μm. Growth of the strain was observed between 10 and 44 °C (optimum 37 °C), in 2–12 % (w/v) NaCl (optimum 3 % NaCl) and between pH 8 and 10·5 (optimum pH 9·5). The novel strain was strictly alkaliphilic, required high concentrations of carbonates in the medium and was capable of utilizing -glucose, fructose, maltose, sucrose, starch and -mannitol. End products of glucose fermentation were H, acetate, ethanol and formate. Strain ASpG1 was resistant to kanamycin and rifampicin, but sensitive to gentamicin, tetracycline and chloramphenicol. The G+C content of its DNA was 58·5 mol%. DNA–DNA hybridization analysis of strain ASpG1 with its most closely related species, Z-7491, revealed a hybridization value of only 48·7 %. On the basis of its physiological and molecular properties, strain ASpG1 appears to represent a novel species of the genus , for which the name is proposed (type strain ASpG1=ATCC BAA-392=DSM 14872).

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2003-05-01
2021-10-26
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