1887

Abstract

A Gram-stain-positive, endospore-forming, rod-shaped bacterium, designated XJ259, was isolated from a cold spring sample from Xinjiang Uyghur Autonomous Region, China. The isolate grew optimally at 20–30 °C and pH 7.3–7.8. Comparative analysis of the 16S rRNA gene sequence showed that isolate XJ259 belonged phylogenetically to the genus , and was most closely related to B538 (with 96.6 % sequence similarity), DS-1 (96.3 %) and Ch-32 (96.1 %), sharing less than 96.0 % sequence similarity with all other members of the genus . Chemotaxonomic analysis revealing menaquinone-7 (MK-7) as the major isoprenoid quinone, diphosphatidylglycerol, phosphatidylethanolamine and two unknown phosphoglycolipids as the major cellular polar lipids, a DNA G+C content of 47.0 mol%, and anteiso-C and C as the major fatty acids supported affiliation of the new isolate to the genus . Based on these data, isolate XJ259 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is XJ259 ( = CGMCC 1.10223  = JCM 16598).

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2011-09-01
2019-12-15
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Neighbour-joining tree of 16S rRNA gene sequences showing phylogenetic relationships between strain XJ259 and all 117 recognized species of the genus . Bootstrap values are shown as percentages of 1000 replicates; only values >50% are shown. Bar, 0.01 substitutions per nucleotide position. [ PDF] (167 KB)

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Transmission electron micrograph showing general morphology and peritrichous flagella of a negatively stained cell of strain XJ259 after growth for 3 days at 25 °C on TYEG agar. Bar, 1.0 µm.

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(a) Light micrograph of a sporulating cell of strain XJ259 . (b) Scanning electron micrograph of a sporulating cell of strain XJ259 ; bar, 2.0 µm. Arrows indicate terminal endospores. Cells were cultured for 3 days at 25 °C on 10-fold diluted TYEG medium supplemented with 5 mg MnSO4 l .

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