1887

Abstract

Anamorphic basidiomycetous yeast strains RS090 and RS092 were isolated from a soil sample collected on Rishiri Island in the Rishiri, Rebun, Sarobetsu National Park, Hokkaido, Japan. As the sequences of the D1/D2 domains of their large-subunit rRNA genes were identical and those of the internal transcribed spacer regions differed in only four bases, we conclude that they belong to a single species with intraspecific diversity. Phylogenetically, this species was related to and , in the Tremellales, Tremellomycetes, Basidiomycota, but was clearly distinct from them. Based on the results of sequence analyses and phenotypic characteristics, we conclude that they belong to a novel species in the genus , for which the name sp. nov. is proposed, with the type strain RS090 ( = JCM 16282  = CBS 11844).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.025254-0
2011-07-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/7/1736.html?itemId=/content/journal/ijsem/10.1099/ijs.0.025254-0&mimeType=html&fmt=ahah

References

  1. Connell L. B. , Redman R. , Craig S. , Scorzetti G. , Iszard M. , Rodriguez R. . ( 2008; ). Diversity of soil yeasts isolated from South Victoria Land, Antarctica. . Microb Ecol 56:, 448–459. [CrossRef].[PubMed].
    [Google Scholar]
  2. Connell L. B. , Redman R. , Rodriguez R. , Barrett A. , Iszard M. , Fonseca Á. . ( 2010; ). Dioszegia antarctica sp. nov. and Dioszegia cryoxerica sp. nov., psychrophilic basidiomycetous yeasts from polar desert soils in Antarctica. . Int J Syst Evol Microbiol 60:, 1466–1472. [CrossRef].[PubMed].
    [Google Scholar]
  3. de García V. , Brizzio S. , Libkind D. , Buzzini P. , van Broock M. . ( 2007; ). Biodiversity of cold-adapted yeasts from glacial meltwater rivers in Patagonia, Argentina. . FEMS Microbiol Ecol 59:, 331–341. [CrossRef].[PubMed].
    [Google Scholar]
  4. Fell J. W. , Boekhout T. , Fonseca A. , Scorzetti G. , Statzell-Tallman A. . ( 2000; ). Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. . Int J Syst Evol Microbiol 50:, 1351–1371.[PubMed].[CrossRef]
    [Google Scholar]
  5. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  6. Inácio J. , Portugal L. , Spencer-Martins I. , Fonseca Á. . ( 2005; ). Phylloplane yeasts from Portugal: seven novel anamorphic species in the Tremellales lineage of the Hymenomycetes (Basidiomycota) producing orange-coloured colonies. . FEMS Yeast Res 5:, 1167–1183. [CrossRef].[PubMed].
    [Google Scholar]
  7. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef].[PubMed].
    [Google Scholar]
  8. Komagata K. , Nakase T. . ( 1967; ). Microbiological studies on frozen foods. V. General properties of yeasts isolated from frozen foods. . J Food Hyg Soc Jpn 8:, 53–57.[CrossRef]
    [Google Scholar]
  9. Kurtzman C. P. , Robnett C. J. . ( 1997; ). Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 5′ end of the large-subunit (26S) ribosomal DNA gene. . J Clin Microbiol 35:, 1216–1223.[PubMed].
    [Google Scholar]
  10. Phaff H. J. , Fell J. W. . ( 1970; ). Cryptococcus Kutzing emend. Phaff et Spencer. . In The Yeasts, A Taxonomic Study, , 2nd edn., pp. 1088–1145. Edited by Lodder J. . . Amsterdam:: North-Holland;.
    [Google Scholar]
  11. Renker C. , Blanke V. , Börstler B. , Heinrichs J. , Buscot F. . ( 2004; ). Diversity of Cryptococcus and Dioszegia yeasts (Basidiomycota) inhabiting arbuscular mycorrhizal roots or spores. . FEMS Yeast Res 4:, 597–603. [CrossRef].[PubMed].
    [Google Scholar]
  12. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed].
    [Google Scholar]
  13. Scorzetti G. , Fell J. W. , Fonseca A. , Statzell-Tallman A. . ( 2002; ). Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. . FEMS Yeast Res 2:, 495–517.[PubMed].[CrossRef]
    [Google Scholar]
  14. Sugita T. , Nakase T. . ( 1999; ). Non-universal usage of the leucine CUG codon and the molecular phylogeny of the genus Candida . . Syst Appl Microbiol 22:, 79–86.[PubMed].[CrossRef]
    [Google Scholar]
  15. Takashima M. , Nakase T. . ( 1999; ). Molecular phylogeny of the genus Cryptococcus and related species based on the sequences of 18S rDNA and internal transcribed spacer regions. . Microbiol Cult Collect 15:, 35–47.
    [Google Scholar]
  16. Takashima M. , Deak T. , Nakase T. . ( 2001; ). Emendation of Dioszegia with redescription of Dioszegia hungarica and two new combinations, Dioszegia aurantiaca and Dioszegia crocea . . J Gen Appl Microbiol 47:, 75–84. [CrossRef].[PubMed].
    [Google Scholar]
  17. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef].[PubMed].
    [Google Scholar]
  18. Thompson J. D. , Higgins D. G. , Gibson T. J. . ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef].[PubMed].
    [Google Scholar]
  19. Vishniac H. S. . ( 1985; ). Yeast biomass in Ross Desert soils: evaluation of quantitation methods and sample transport effects. . Antarct J US 19:, 174–176.
    [Google Scholar]
  20. Vishniac H. S. . ( 2006a; ). A multivariate analysis of soil yeasts isolated from a latitudinal gradient. . Microb Ecol 52:, 90–103. [CrossRef].[PubMed].
    [Google Scholar]
  21. Vishniac H. S. . ( 2006b; ). Yeast biodiversity in the Antarctic. . In Biodiversity and Ecophysiology of Yeasts, pp. 419–440. Edited by Péter G. , Rosa C. . . Heidelberg:: Springer;. [CrossRef].
    [Google Scholar]
  22. Wang Q. M. , Jia J. H. , Bai F. Y. . ( 2008; ). Diversity of basidiomycetous phylloplane yeasts belonging to the genus Dioszegia (Tremellales) and description of Dioszegia athyri sp. nov., Dioszegia butyracea sp. nov. and Dioszegia xingshanensis sp. nov.. Antonie van Leeuwenhoek 93:, 391–399. [CrossRef].[PubMed].
    [Google Scholar]
  23. Yarrow D. . ( 1998; ). Methods for the isolation, maintenance and identification of yeasts. . In The Yeasts, A Taxonomic Study, , 4th edn., pp. 77–100. Edited by Kurtzman C. P. , Fell J. W. . . Amsterdam:: Elsevier;. [CrossRef].
    [Google Scholar]
  24. Zsolt J. . ( 1957; ). Egy új élesztö: Dioszegia hungarica nov. gen. et sp.. Bot Kozl 47:, 63–66 (in Hungarian).
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.025254-0
Loading
/content/journal/ijsem/10.1099/ijs.0.025254-0
Loading

Data & Media loading...

Supplements

vol. , part 7, pp. 1736 - 1739

Neighbour-joining tree of sp. nov. and related species based on the combined ITS and D1/D2 domain of the LSU rRNA gene sequence. [PDF](37 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error