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A novel strictly aerobic, heterotrophic, mesophilic bacterium, strain Mb8T, was isolated from soil in Sapporo City, Hokkaido, Japan. The G+C content of strain Mb8T was 66·0 mol%. It had mycolic acids with 44–52 carbon atoms and C16 : 0 and C18 : 1 (9) as the major fatty acids. The major isoprenoid quinone was MK-8(H2). The cell wall contained meso-diaminopimelic acid, arabinose and galactose. 16S rDNA, chemotaxonomic and morphological data indicated that this strain clearly belonged to the genus Rhodococcus. Based on phenotypic properties and DNA–DNA hybridization data, strain Mb8T (=JCM 11308T=NCIMB 13903T) has been assigned to the genus Rhodococcus as the type strain of Rhodococcus tukisamuensis sp. nov.
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