1887

Abstract

A novel strain, designated HY-50R, isolated from soil of a Euphrates poplar () forest in Xinjiang, China, was characterized using a polyphasic taxonomic approach. Cells of the isolate were Gram-reaction-negative, strictly aerobic, rod-shaped, non-motile, oxidase-negative and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate was a member of the phylum , its closest relatives being THYL-44 (93.6 % similarity), Gsoil 643 (93.5 %), IAM 15098 (93.3 %) and Gsoil 492 (93.2 %). The major fatty acids were iso-C G (11.7 %), iso-C (19.6 %) and iso-C 3-OH (19.3 %). The predominant menaquinone of strain HY-50R was MK-7 and the genomic DNA G+C content was 46.8 mol%. Flexirubin-type pigments were not produced. Based on phylogenetic evidence and the results of phenotypic, genotypic and chemotaxonomic analysis, strain HY-50R represents a novel species of a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain is HY-50R ( = CCTCC AB 208255  = NRRL B-59222).

Funding
This study was supported by the:
  • R & D Infrastructure and Facility Development Program from the Ministry of Science and Technology of the People’s Republic of China (Award 2005DKA21208)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.025221-0
2011-07-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/7/1554.html?itemId=/content/journal/ijsem/10.1099/ijs.0.025221-0&mimeType=html&fmt=ahah

References

  1. An D. S., Lee H. G., Im W. T., Liu Q. M., Lee S. T. 2007; Segetibacter koreensis gen. nov., sp. nov., a novel member of the phylum Bacteroidetes, isolated from the soil of a ginseng field in South Korea. Int J Syst Evol Microbiol 57:1828–1833 [View Article][PubMed]
    [Google Scholar]
  2. Choi D. H., Zhang G. I., Noh J. H., Kim W. S., Cho B. C. 2009; Gracilimonas tropica gen. nov., sp. nov., isolated from a Synechococcus culture. Int J Syst Evol Microbiol 59:1167–1172 [CrossRef]
    [Google Scholar]
  3. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  5. Fitch W. M. 1971; Towards defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  6. Garrity G. M., Holt J. G. 2001; The road map to the Manual . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 1 pp. 119–166 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer;
    [Google Scholar]
  7. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  8. Güde H. 1980; Occurrence of cytophagas in sewage plants. Appl Environ Microbiol 39:756–763[PubMed]
    [Google Scholar]
  9. Kämpfer P., Young C. C., Sridhar K. R., Arun A. B., Lai W. A., Shen F. T., Rekha P. D. 2006; Transfer of [Flexibacter] sancti, [Flexibacter] filiformis, [Flexibacter] japonensis and [Cytophaga] arvensicola to the genus Chitinophaga and description of Chitinophaga skermanii sp. nov.. Int J Syst Evol Microbiol 56:2223–2228 [View Article][PubMed]
    [Google Scholar]
  10. Kämpfer P., Lodders N., Falsen E. 2011; Hydrotalea flava gen. nov. sp. nov., a new species of the phylum Bacteriodetes and allocation of the genera Chitinophaga, Sediminibacterium, Lacibacter, Flavihumibacter, Flavisolibacter, Niabella, Niastella, Segetibacter, Parasegetibacter, Terrimonas, Ferruginibacter, Filimonas and Hydrotalea to the family Chitinophagaceae fam. nov.. Int J Syst Evol Microbiol 61:518–523 [View Article]
    [Google Scholar]
  11. Kim B. Y., Weon H. Y., Yoo S. H., Hong S. B., Kwon S. W., Stackebrandt E., Go S. J. 2007; Niabella aurantiaca gen. nov., sp. nov., isolated from a greenhouse soil in Korea. Int J Syst Evol Microbiol 57:538–541 [View Article][PubMed]
    [Google Scholar]
  12. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  13. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp. 115–147 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  14. Lim J. H., Baek S.-H., Lee S.-T. 2009; Ferruginibacter alkalilentus gen. nov., sp. nov. and Ferruginibacter lapsinanis sp. nov., novel members of the family ‘Chitinophagaceae’ in the phylum Bacteroidetes, isolated from freshwater sediment. Int J Syst Bacteriol 59:2394–2399 [CrossRef]
    [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  16. MIDI 1999; Sherlock microbial identification system, operating manual, version 3.0. Newark, DE: MIDI, Inc.;
  17. Qu J. H., Yuan H. L. 2008; Sediminibacterium salmoneum gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from sediment of a eutrophic reservoir. Int J Syst Evol Microbiol 58:2191–2194 [View Article][PubMed]
    [Google Scholar]
  18. Qu J. H., Yuan H. L., Yang J. S., Li H. F., Chen N. 2009; Lacibacter cauensis gen. nov., sp. nov., a novel member of the phylum Bacteroidetes isolated from sediment of a eutrophic lake. Int J Syst Evol Microbiol 59:1153–1157 [View Article][PubMed]
    [Google Scholar]
  19. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  20. Schenkel E., Berlaimont V., Dubois J., Helson-Cambier M., Hanocq M. 1995; Improved high-performance liquid chromatographic method for the determination of polyamines as their benzoylated derivatives: application to P388 cancer cells. J Chromatogr B Biomed Appl 668:189–197 [View Article][PubMed]
    [Google Scholar]
  21. Sheu S.-Y., Cho N.-T., Arun A. B., Chen W.-M. 2010; Terrimonas aquatica sp. nov., isolated from a freshwater spring. Int J Syst Evol Microbiol 60:2705–2709 [View Article][PubMed]
    [Google Scholar]
  22. Shiratori H., Tagami Y., Morishita T., Kamihara Y., Beppu T., Ueda K. 2009; Filimonas lacunae gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from fresh water. Int J Syst Evol Microbiol 59:1137–1142 [View Article][PubMed]
    [Google Scholar]
  23. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Woods W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  24. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  25. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  26. Urios L., Agogué H., Lesongeur F., Stackebrandt E., Lebaron P. 2006; Balneola vulgaris gen. nov., sp. nov., a member of the phylum Bacteroidetes from the north-western Mediterranean Sea. Int J Syst Evol Microbiol 56:1883–1887 [CrossRef]
    [Google Scholar]
  27. Weon H. Y., Kim B. Y., Yoo S. H., Lee S. Y., Kwon S. W., Go S. J., Stackebrandt E. 2006; Niastella koreensis gen. nov., sp. nov. and Niastella yeongjuensis sp. nov., novel members of the phylum Bacteroidetes, isolated from soil cultivated with Korean ginseng. Int J Syst Evol Microbiol 56:1777–1782 [View Article][PubMed]
    [Google Scholar]
  28. Wilson K. 1987; Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology pp. 241–245 Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. New York: Greene Publishing and Wiley-Interscience;
    [Google Scholar]
  29. Xie C. H., Yokota A. 2003; Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49:345–349 [View Article][PubMed]
    [Google Scholar]
  30. Xie C. H., Yokota A. 2006; Reclassification of [Flavobacterium] ferrugineum as Terrimonas ferruginea gen. nov., comb. nov., and description of Terrimonas lutea sp. nov., isolated from soil. Int J Syst Evol Microbiol 56:1117–1121 [View Article][PubMed]
    [Google Scholar]
  31. Xu P., Li W.-J., Tang S.-K., Zhang Y.-Q., Chen G.-Z., Chen H.-H., Xu L.-H., Jiang C.-L. 2005; Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55:1149–1153 [View Article][PubMed]
    [Google Scholar]
  32. Yoon M. H., Im W. T. 2007; Flavisolibacter ginsengiterrae gen. nov., sp. nov. and Flavisolibacter ginsengisoli sp. nov., isolated from ginseng cultivating soil. Int J Syst Evol Microbiol 57:1834–1839 [View Article][PubMed]
    [Google Scholar]
  33. Zhang K. D., Tang Y. L., Zhang L., Dai J., Wang Y., Luo X. S., Liu M., Luo G. C., Fang C. X. 2009; Parasegetibacter luojiensis gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from a forest soil. Int J Syst Evol Microbiol 59:3058–3062 [View Article][PubMed]
    [Google Scholar]
  34. Zhang N. N., Qu J.-H., Yuan H.-L., Sun Y. M., Yang J.-S. 2010; Flavihumibacter petaseus gen. nov., sp. nov., isolated from soil of a subtropical rainforest. Int J Syst Evol Microbiol 60:1609–1612 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.025221-0
Loading
/content/journal/ijsem/10.1099/ijs.0.025221-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error