1887

Abstract

A novel strain, designated HY-50R, isolated from soil of a Euphrates poplar () forest in Xinjiang, China, was characterized using a polyphasic taxonomic approach. Cells of the isolate were Gram-reaction-negative, strictly aerobic, rod-shaped, non-motile, oxidase-negative and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate was a member of the phylum , its closest relatives being THYL-44 (93.6 % similarity), Gsoil 643 (93.5 %), IAM 15098 (93.3 %) and Gsoil 492 (93.2 %). The major fatty acids were iso-C G (11.7 %), iso-C (19.6 %) and iso-C 3-OH (19.3 %). The predominant menaquinone of strain HY-50R was MK-7 and the genomic DNA G+C content was 46.8 mol%. Flexirubin-type pigments were not produced. Based on phylogenetic evidence and the results of phenotypic, genotypic and chemotaxonomic analysis, strain HY-50R represents a novel species of a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain is HY-50R ( = CCTCC AB 208255  = NRRL B-59222).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.025221-0
2011-07-01
2019-09-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/7/1554.html?itemId=/content/journal/ijsem/10.1099/ijs.0.025221-0&mimeType=html&fmt=ahah

References

  1. An D. S. , Lee H. G. , Im W. T. , Liu Q. M. , Lee S. T. . ( 2007; ). Segetibacter koreensis gen. nov., sp. nov., a novel member of the phylum Bacteroidetes, isolated from the soil of a ginseng field in South Korea. . Int J Syst Evol Microbiol 57:, 1828–1833. [CrossRef].[PubMed]
    [Google Scholar]
  2. Choi D. H. , Zhang G. I. , Noh J. H. , Kim W. S. , Cho B. C. . ( 2009; ). Gracilimonas tropica gen. nov., sp. nov., isolated from a Synechococcus culture. . Int J Syst Evol Microbiol 59:, 1167–1172.[CrossRef]
    [Google Scholar]
  3. Chun J. , Lee J.-H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef].[PubMed]
    [Google Scholar]
  4. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  5. Fitch W. M. . ( 1971; ). Towards defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  6. Garrity G. M. , Holt J. G. . ( 2001; ). The road map to the Manual . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 1, pp. 119–166. Edited by Boone D. R. , Castenholz R. W. , Garrity G. M. . . New York:: Springer;.
    [Google Scholar]
  7. Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  8. Güde H. . ( 1980; ). Occurrence of cytophagas in sewage plants. . Appl Environ Microbiol 39:, 756–763.[PubMed]
    [Google Scholar]
  9. Kämpfer P. , Young C. C. , Sridhar K. R. , Arun A. B. , Lai W. A. , Shen F. T. , Rekha P. D. . ( 2006; ). Transfer of [Flexibacter] sancti, [Flexibacter] filiformis, [Flexibacter] japonensis and [Cytophaga] arvensicola to the genus Chitinophaga and description of Chitinophaga skermanii sp. nov.. Int J Syst Evol Microbiol 56:, 2223–2228. [CrossRef].[PubMed]
    [Google Scholar]
  10. Kämpfer P. , Lodders N. , Falsen E. . ( 2011; ). Hydrotalea flava gen. nov. sp. nov., a new species of the phylum Bacteriodetes and allocation of the genera Chitinophaga, Sediminibacterium, Lacibacter, Flavihumibacter, Flavisolibacter, Niabella, Niastella, Segetibacter, Parasegetibacter, Terrimonas, Ferruginibacter, Filimonas and Hydrotalea to the family Chitinophagaceae fam. nov.. Int J Syst Evol Microbiol 61:, 518–523. [CrossRef]
    [Google Scholar]
  11. Kim B. Y. , Weon H. Y. , Yoo S. H. , Hong S. B. , Kwon S. W. , Stackebrandt E. , Go S. J. . ( 2007; ). Niabella aurantiaca gen. nov., sp. nov., isolated from a greenhouse soil in Korea. . Int J Syst Evol Microbiol 57:, 538–541. [CrossRef].[PubMed]
    [Google Scholar]
  12. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef].[PubMed]
    [Google Scholar]
  13. Lane D. J. . ( 1991; ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–147. Edited by Stackebrandt E. , Goodfellow M. . . Chichester:: Wiley;.
    [Google Scholar]
  14. Lim J. H. , Baek S.-H. , Lee S.-T. . ( 2009; ). Ferruginibacter alkalilentus gen. nov., sp. nov. and Ferruginibacter lapsinanis sp. nov., novel members of the family ‘Chitinophagaceae’ in the phylum Bacteroidetes, isolated from freshwater sediment. . Int J Syst Bacteriol 59:, 2394–2399.[CrossRef]
    [Google Scholar]
  15. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  16. MIDI ( 1999; ). Sherlock microbial identification system, operating manual, version 3.0. . Newark, DE:: MIDI, Inc.;
  17. Qu J. H. , Yuan H. L. . ( 2008; ). Sediminibacterium salmoneum gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from sediment of a eutrophic reservoir. . Int J Syst Evol Microbiol 58:, 2191–2194. [CrossRef].[PubMed]
    [Google Scholar]
  18. Qu J. H. , Yuan H. L. , Yang J. S. , Li H. F. , Chen N. . ( 2009; ). Lacibacter cauensis gen. nov., sp. nov., a novel member of the phylum Bacteroidetes isolated from sediment of a eutrophic lake. . Int J Syst Evol Microbiol 59:, 1153–1157. [CrossRef].[PubMed]
    [Google Scholar]
  19. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  20. Schenkel E. , Berlaimont V. , Dubois J. , Helson-Cambier M. , Hanocq M. . ( 1995; ). Improved high-performance liquid chromatographic method for the determination of polyamines as their benzoylated derivatives: application to P388 cancer cells. . J Chromatogr B Biomed Appl 668:, 189–197. [CrossRef].[PubMed]
    [Google Scholar]
  21. Sheu S.-Y. , Cho N.-T. , Arun A. B. , Chen W.-M. . ( 2010; ). Terrimonas aquatica sp. nov., isolated from a freshwater spring. . Int J Syst Evol Microbiol 60:, 2705–2709. [CrossRef].[PubMed]
    [Google Scholar]
  22. Shiratori H. , Tagami Y. , Morishita T. , Kamihara Y. , Beppu T. , Ueda K. . ( 2009; ). Filimonas lacunae gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from fresh water. . Int J Syst Evol Microbiol 59:, 1137–1142. [CrossRef].[PubMed]
    [Google Scholar]
  23. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Woods W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  24. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef].[PubMed]
    [Google Scholar]
  25. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef].[PubMed]
    [Google Scholar]
  26. Urios L. , Agogué H. , Lesongeur F. , Stackebrandt E. , Lebaron P. . ( 2006; ). Balneola vulgaris gen. nov., sp. nov., a member of the phylum Bacteroidetes from the north-western Mediterranean Sea. . Int J Syst Evol Microbiol 56:, 1883–1887.[CrossRef]
    [Google Scholar]
  27. Weon H. Y. , Kim B. Y. , Yoo S. H. , Lee S. Y. , Kwon S. W. , Go S. J. , Stackebrandt E. . ( 2006; ). Niastella koreensis gen. nov., sp. nov. and Niastella yeongjuensis sp. nov., novel members of the phylum Bacteroidetes, isolated from soil cultivated with Korean ginseng. . Int J Syst Evol Microbiol 56:, 1777–1782. [CrossRef].[PubMed]
    [Google Scholar]
  28. Wilson K. . ( 1987; ). Preparation of genomic DNA from bacteria. . In Current Protocols in Molecular Biology, pp. 241–245. Edited by Ausubel F. M. , Brent R. , Kingston R. E. , Moore D. D. , Seidman J. G. , Smith J. A. , Struhl K. . . New York:: Greene Publishing and Wiley-Interscience;.
    [Google Scholar]
  29. Xie C. H. , Yokota A. . ( 2003; ). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. . J Gen Appl Microbiol 49:, 345–349. [CrossRef].[PubMed]
    [Google Scholar]
  30. Xie C. H. , Yokota A. . ( 2006; ). Reclassification of [Flavobacterium] ferrugineum as Terrimonas ferruginea gen. nov., comb. nov., and description of Terrimonas lutea sp. nov., isolated from soil. . Int J Syst Evol Microbiol 56:, 1117–1121. [CrossRef].[PubMed]
    [Google Scholar]
  31. Xu P. , Li W.-J. , Tang S.-K. , Zhang Y.-Q. , Chen G.-Z. , Chen H.-H. , Xu L.-H. , Jiang C.-L. . ( 2005; ). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol 55:, 1149–1153. [CrossRef].[PubMed]
    [Google Scholar]
  32. Yoon M. H. , Im W. T. . ( 2007; ). Flavisolibacter ginsengiterrae gen. nov., sp. nov. and Flavisolibacter ginsengisoli sp. nov., isolated from ginseng cultivating soil. . Int J Syst Evol Microbiol 57:, 1834–1839. [CrossRef].[PubMed]
    [Google Scholar]
  33. Zhang K. D. , Tang Y. L. , Zhang L. , Dai J. , Wang Y. , Luo X. S. , Liu M. , Luo G. C. , Fang C. X. . ( 2009; ). Parasegetibacter luojiensis gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from a forest soil. . Int J Syst Evol Microbiol 59:, 3058–3062. [CrossRef].[PubMed]
    [Google Scholar]
  34. Zhang N. N. , Qu J.-H. , Yuan H.-L. , Sun Y. M. , Yang J.-S. . ( 2010; ). Flavihumibacter petaseus gen. nov., sp. nov., isolated from soil of a subtropical rainforest. . Int J Syst Evol Microbiol 60:, 1609–1612. [CrossRef].[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.025221-0
Loading
/content/journal/ijsem/10.1099/ijs.0.025221-0
Loading

Data & Media loading...

vol. , part 7, pp. 1554 - 1560

Phylogenetic tree reconstructed using 16S rRNA gene sequences under the maximum-parsimony criterion performed by using the close-neighbour-interchange search option. [PDF](223KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error