%0 Journal Article %A Busse, Hans-Jürgen %A Zlamala, Christian %A Buczolits, Sandra %A Lubitz, Werner %A Kämpfer, Peter %A Takeuchi, Mariko %T Promicromonospora vindobonensis sp. nov. and Promicromonospora aerolata sp. nov., isolated from the air in the medieval ‘Virgilkapelle’ in Vienna %D 2003 %J International Journal of Systematic and Evolutionary Microbiology, %V 53 %N 5 %P 1503-1507 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.02522-0 %I Microbiology Society, %X Two airborne bacterial isolates designated V45T and V54AT were characterized in order to determine their taxonomic position. 16S rDNA sequence analysis showed that the two isolates shared 98·1 % sequence similarity. Highest sequence similarities (98·0–98·5 %) were found to Promicromonospora citrea DSM 43110T and Promicromonospora sukumoe IFO 14650T. Detection of a quinone system with the predominant compound MK-9(H4), a polar lipid pattern containing phosphatidylglycerol, a fatty acid profile with the predominant acids C15 : 0 iso and C15 : 0 anteiso and the diagnostic cell-wall diamino acid l-lysine supported the assignment of the novel isolates to the genus Promicromonospora. The two isolates could be distinguished from P. sukumoe by the presence of glycine in the peptidoglycan, and the detection of the cell-wall sugar galactose differentiates them from the two established species of the genus Promicromonospora. Each of the two isolates displayed a unique biochemical profile. Results from DNA–DNA hybridizations clearly demonstrated that V45T and V54AT represent separate species. Based on these data, it is proposed that V45T (=IFO 16525T=CCM 7044T) and V54AT (=IFO 16526T =CCM 7043T) be classified as the type strains of two novel Promicromonospora species, for which the names Promicromonospora vindobonensis sp. nov. and Promicromonospora aerolata sp. nov. are proposed. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.02522-0