1887

Abstract

Previous studies using the nuclear SSU rDNA have indicated that the photosynthetic euglenoids are a monophyletic group; however, some of the genera within the photosynthetic lineage are not monophyletic. To test these results further, evolutionary relationships among the photosynthetic genera were investigated by obtaining partial LSU nuclear rDNA sequences. Taxa from each of the external clades of the SSU rDNA-based phylogeny were chosen to create a combined dataset and to compare the individual LSU and SSU rDNA datasets. Conserved areas of the aligned sequences for both the LSU and SSU rDNA were used to generate parsimony, log-det, maximum-likelihood and Bayesian trees. The SSU and LSU rDNA consistently generated the same seven terminal clades; however, the relationship among those clades varied depending on the type of analysis and the dataset used. The combined dataset generated a more robust phylogeny, but the relationships among clades still varied. The addition of the LSU rDNA dataset to the euglenophyte phylogeny supports the view that the genera , and are not monophyletic and substantiates the existence of several well-supported clades. A secondary structural model for the D2 region of the LSU rDNA was proposed on the basis of compensatory base changes found in the alignment.

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2003-07-01
2019-12-05
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Secondary structure of for the portion of the LSU rDNA sequenced in this analysis. [PDF](11 KB)

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Secondary structural alignment for all 34 taxa sequenced in this analysis. TVS, var. ; P. pleur. v. triquetra, var. . Numbers in parentheses after organism names indicate culture collection numbers in order to differentiate identical taxa. Within the sequence alignment, parentheses indicate a stem sequence narrowly separated from its complementary sequence. A pair of straight lines around a sequence notes a stem region that is separated from its complementary sequence by other hydrogen-bonded stems within the alignment. In both cases, complementary base pairs are underlined. [PDF](150 KB)

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SSU rDNA. supplementary_SSU_rDNA_nexus.NEX(56 KB)

LSU rDNA. supplementary_LSU_rDNA_nexus.NEX(27 KB)

Combined dataset. supplementary_combo_nexus.NEX(81 KB)

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