sp. nov., isolated from coastal water Free

Abstract

A facultatively anaerobic marine gammaproteobacterium, designated strain M46, was isolated from Mediterranean seawater at Malvarrosa beach, Valencia, Spain. The strain was characterized by using a polyphasic approach and was found to be situated within the genus in the family . Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46 was closely related to . CECT 7644, CECT 5860 and CECT 7641, showing sequence similarities of 96.8, 96.4 and 96.2 %, respectively. According to the results of phylogenetic analyses based on and gene sequences, the most closely related taxon was CECT 7641 with 87.4 and 85.0 % sequence similarity, respectively. Regardless of the gene used in phylogenetic analysis, strain M46 always formed a separate and stable clade containing these three species of the genus . Strain M46 was not luminescent and produced a diffusible brown pigment. It required NaCl to grow, reduced nitrate to nitrite and oxidized a small number of substrates in Biolog GN plates. Strain M46 was positive for arginine dihydrolase (ADH), β-galactosidase, aesculin hydrolysis and DNase activity. In API ZYM tests, the novel strain was positive for alkaline phosphatase, leucine arylamidase and acidic phosphatase activities. The major cellular fatty acids were unsaturated C and C, as in other members of the genus , but their relative amounts and the presence or absence of other fatty acids differentiated strain M46 from its closest relatives. Based on the results of this polyphasic taxonomic study, strain M46 represents a novel species of the genus , for which the name is proposed. The type strain is M46 ( = CECT 7614  = KCTC 23057).

Funding
This study was supported by the:
  • Spanish Ministerio de Educación y Ciencia (Award CGL2005-02292)
  • Spanish Ministry of Education and Science
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2011-07-01
2024-03-29
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References

  1. Ast J. C., Dunlap P. V. 2005; Phylogenetic resolution and habitat specificity of members of the Photobacterium phosphoreum species group. Environ Microbiol 7:1641–1654 [View Article][PubMed]
    [Google Scholar]
  2. Baumann P., Baumann L. 1981; The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas and Alcaligenes . In The Prokaryotes vol. 2 pp. 1302–1331 Edited by Starr M. P., Stolp H., Trueper H. G., Balows A., Schleger H. Berlin, Heidelberg: Springer;
    [Google Scholar]
  3. Chimetto L. A., Cleenwerck I., Thompson C. C., Brocchi M., Willems A., De Vos P., Thompson F. L. 2010; Photobacterium jeanii sp. nov., isolated from corals and zoanthids. Int J Syst Evol Microbiol 60:2843–2848 [View Article][PubMed]
    [Google Scholar]
  4. Dunlap P. V., Ast J. C. 2005; Genomic and phylogenetic characterization of luminous bacteria symbiotic with the deep-sea fish Chlorophthalmus albatrossis (Aulopiformes: Chlorophthalmidae). Appl Environ Microbiol 71:930–939 [View Article][PubMed]
    [Google Scholar]
  5. Farmer J. J. III, Hickman-Brenner F. W. 2006; The genera Vibrio and Photobacterium . In The Prokaryotes, 3rd edn. pp. 508–563 Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. New York: Springer; [View Article]
    [Google Scholar]
  6. Jung S. Y., Jung Y. T., Oh T. K., Yoon J. H. 2007; Photobacterium lutimaris sp. nov., isolated from a tidal flat sediment in Korea. Int J Syst Evol Microbiol 57:332–336 [View Article][PubMed]
    [Google Scholar]
  7. Kim Y. O., Kim K. K., Park S., Kang S. J., Lee J. H., Lee S. J., Oh T. K., Yoon J. H. 2010; Photobacterium gaetbulicola sp. nov., a lipolytic bacterium isolated from a tidal flat sediment. Int J Syst Evol Microbiol 60:2587–2591 [View Article][PubMed]
    [Google Scholar]
  8. Lucena T., Pascual J., Garay E., Arahal D. R., Macián M. C., Pujalte M. J. 2010; Haliea mediterranea sp. nov., a marine gammaproteobacterium. Int J Syst Evol Microbiol 60:1844–1848 [View Article][PubMed]
    [Google Scholar]
  9. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. et al. 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [View Article][PubMed]
    [Google Scholar]
  10. Macián M. C., Ludwig W., Aznar R., Grimont P. A., Schleifer K. H., Garay E., Pujalte M. J. 2001; Vibrio lentus sp. nov., isolated from Mediterranean oysters. Int J Syst Evol Microbiol 51:1449–1456[PubMed] [CrossRef]
    [Google Scholar]
  11. Macián M. C., Arahal D. R., Garay E., Ludwig W., Schleifer K.-H., Pujalte M. J. 2005; Thalassobacter stenotrophicus gen. nov., sp. nov., a novel marine α-proteobacterium isolated from Mediterranean sea water. Int J Syst Evol Microbiol 55:105–110 [View Article][PubMed]
    [Google Scholar]
  12. MIDI 2008Sherlock Microbial Identification System Operating Manual, version 6.1 Newark, DE: MIDI Inc;
  13. Park Y. D., Baik K. S., Seong C. N., Bae K. S., Kim S., Chun J. 2006; Photobacterium ganghwense sp. nov., a halophilic bacterium isolated from sea water. Int J Syst Evol Microbiol 56:745–749 [View Article][PubMed]
    [Google Scholar]
  14. Pascual J., Macián M. C., Arahal D. R., Garay E., Pujalte M. J. 2010; Multilocus sequence analysis of the central clade of the genus Vibrio by using the 16S rRNA, recA, pyrH, rpoD, gyrB, rctB and toxR genes. Int J Syst Evol Microbiol 60:154–165 [View Article][PubMed]
    [Google Scholar]
  15. Rivas R., García-Fraile P., Mateos P. F., Martínez-Molina E., Velázquez E. 2006; Photobacterium halotolerans sp. nov., isolated from Lake Martel in Spain. Int J Syst Evol Microbiol 56:1067–1071 [View Article][PubMed]
    [Google Scholar]
  16. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc;
  17. Stackebrandt E., Goebel J. 1994; Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  18. Wimpee C. F., Nadeau T. L., Nealson K. H. 1991; Development of species-specific hybridization probes for marine luminous bacteria by using in vitro DNA amplification. Appl Environ Microbiol 57:1319–1324[PubMed]
    [Google Scholar]
  19. Yoshizawa S., Wada M., Kita-Tsukamoto K., Yokota A., Kogure K. 2009; Photobacterium aquimaris sp. nov., a luminous marine bacterium isolated from seawater. Int J Syst Evol Microbiol 59:1438–1442 [View Article][PubMed]
    [Google Scholar]
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