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Abstract

A facultatively anaerobic marine gammaproteobacterium, designated strain M46, was isolated from Mediterranean seawater at Malvarrosa beach, Valencia, Spain. The strain was characterized by using a polyphasic approach and was found to be situated within the genus in the family . Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M46 was closely related to . CECT 7644, CECT 5860 and CECT 7641, showing sequence similarities of 96.8, 96.4 and 96.2 %, respectively. According to the results of phylogenetic analyses based on and gene sequences, the most closely related taxon was CECT 7641 with 87.4 and 85.0 % sequence similarity, respectively. Regardless of the gene used in phylogenetic analysis, strain M46 always formed a separate and stable clade containing these three species of the genus . Strain M46 was not luminescent and produced a diffusible brown pigment. It required NaCl to grow, reduced nitrate to nitrite and oxidized a small number of substrates in Biolog GN plates. Strain M46 was positive for arginine dihydrolase (ADH), β-galactosidase, aesculin hydrolysis and DNase activity. In API ZYM tests, the novel strain was positive for alkaline phosphatase, leucine arylamidase and acidic phosphatase activities. The major cellular fatty acids were unsaturated C and C, as in other members of the genus , but their relative amounts and the presence or absence of other fatty acids differentiated strain M46 from its closest relatives. Based on the results of this polyphasic taxonomic study, strain M46 represents a novel species of the genus , for which the name is proposed. The type strain is M46 ( = CECT 7614  = KCTC 23057).

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2011-07-01
2020-01-26
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Supplements

Supplementary Fig. S1. Cultures of CECT 7644 (a), CECT 5860 (b), CECT 7614 (c) and CECT 7641 (d) grown on LM at 28 °C for 2 days. and produce diffusible brown and pink pigments, respectively.

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Maximum-parsimony phylogenetic trees based on almost complete 16S rRNA (a), partial (b), partial (c) and concatenated (d) gene sequences of sp. nov. CECT 7614 and related species. [PDF]

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Maximum-likelihood phylogenetic trees based on almost complete 16S rRNA (a), partial (b), partial (c) and concatenated (d) gene sequences of sp. nov. CECT 7614 and related species. [PDF]

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Substrates oxidized in Biolog GN microplates by M46 and closely related species of the genus [PDF]

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