1887

Abstract

A marine bacterium producing protease inhibitors was isolated from neritic sea water and was studied phenotypically, genotypically and phylogenetically. This bacterium (strain B-10-31) produced three types of protease inhibitor, namely, marinostatin, monastatin and leupeptin, which were considerably different in terms of their chemical structure and properties. Strain B-10-31 was a rod-shaped, non-spore-forming, Gram-negative, strictly aerobic bacterium that was motile by means of one polar flagellum. The strain required Na for growth and exhibited optimal growth at 27 °C, pH 8·0 and 2 % (w/v) NaCl. It utilized various substrates, such as -glucose, maltose, maltotriose, -acetylglucosamine, -threonine, -serine, -arginine, -proline, --alanine and -glutamate, as the sole energy source. Ubiquinone-8 was the major respiratory quinone. The major fatty acids were C, C 7, C 9 and C 7. The G+C content of the DNA of strain B-10-31 was 42·0 mol%. Phylogenetic analysis, based on 16S rDNA sequences, showed that the strain clustered in the -. The aerobic marine bacterium was the species most closely related to the new isolate (90·4 % 16S rDNA sequence similarity); other described species in the - cluster showed low levels of sequence similarity with strain B-10-31 (<90 %). Based on the above results, it is proposed that the novel marine bacterium should be classified as a new species, for which the name (type strain B-10-31=JCM 11461=DSM 14643) is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02516-0
2003-11-01
2021-09-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/53/6/ijs531807.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02516-0&mimeType=html&fmt=ahah

References

  1. Baumann P., Baumann L., Mandel M. 1971; Taxonomy of marine bacteria: the genus Beneckea . J Bacteriol 85:268–294
    [Google Scholar]
  2. Baumann P., Gauthier M. J., Baumann L. 1984; Genus Alteromonas Baumann, Baumann, Mandel and Allen 1972, 418AL . In Bergey's Manual of Systematic Bacteriology vol 1 pp 343–352Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  3. Bowman J. P., McCammon S. A., Brown J. L., McMeekin T. A. 1998; Glaciecola punicea gen. nov., sp. nov. and Glaciecola pallidula gen. nov. sp. nov.: psychrophilic bacteria from Antarctic sea-ice habitats. Int J Syst Bacteriol 48:1213–1222 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c Department of Genetics, University of Washington; Seattle, USA:
    [Google Scholar]
  5. Gauthier G., Gauthier M., Christen R. 1995; Phylogenetic analysis of the genera Alteromonas , Shewanella , and Moritella using genes coding for small-subunit rRNA sequences and division of the genus Alteromonas into two genera, Alteromonas (emended) and Pseudoalteromonas gen. nov., and proposal of twelve new species combinations. Int J Syst Bacteriol 45:755–761 [CrossRef]
    [Google Scholar]
  6. Hamato N., Takano R., Kamei-Hayashi K., Imada C., Hara S. 1992; Leupeptins produced by the marine Alteromonas sp. B-10-31. Biosci Biotechnol Biochem 110:856–858
    [Google Scholar]
  7. Hiraishi A., Ueda Y., Ishihara J., Mori T. 1996; Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42:457–469 [CrossRef]
    [Google Scholar]
  8. Imada C., Simidu U., Taga N. 1985a; Isolation and characterization of marine bacteria producing alkaline protease inhibitor. Bull Jpn Soc Sci Fish 51:799–803 [CrossRef]
    [Google Scholar]
  9. Imada C., Maeda M., Taga N. 1985b; Purification and characterization of the protease inhibitor ‘monastatin’ from a marine Alteromonas sp. with reference to inhibition of the protease produced by a bacterium pathogenic to fish. Can J Microbiol 31:1089–1094 [CrossRef]
    [Google Scholar]
  10. Imada C., Taga N., Maeda M. 1985c; Cultivation conditions for subtilisin inhibitor-producing bacterium and general properties of the inhibitor ‘marinostatin’. Bull Jpn Soc Sci Fish 51:805–810 [CrossRef]
    [Google Scholar]
  11. Imada C., Maeda M., Hara S., Taga N., Simidu U. 1986a; Purification and characterization of the subtilisin inhibitor ‘marinostatin’ produced by marine Alteromonas sp. J Appl Bacteriol 60:469–476 [CrossRef]
    [Google Scholar]
  12. Imada C., Hara S., Maeda M., Simidu U. 1986b; Amino acid sequences of marinostatins C-1 and C-2 from marine Alteromonas sp. Bull Jpn Soc Sci Fish 52:1455–1459 [CrossRef]
    [Google Scholar]
  13. Isnansetyo A., Kamei Y. 2003; Pseudoalteromonas phenolica sp. nov., a novel marine bacterium that produces phenolic anti-methicillin-resistant Staphylococcus aureus substances. Int J Syst Evol Microbiol 53:583–588 [CrossRef]
    [Google Scholar]
  14. Ivanova E. P., Romanenko L. A., Chun J. 7 other authors 2000; Idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the Pacific Ocean, including descriptions of two species, Idiomarina abyssalis sp. nov. and Idiomarina zobellii sp. nov. Int J Syst Evol Microbiol 50:901–907 [CrossRef]
    [Google Scholar]
  15. Ivanova E. P., Shevchenko L. S., Sawabe T., Lysenko A. M., Svetashev V. I., Gorshkova N. M., Satomi M., Christen R., Mikhailov V. V. 2002; Pseudoalteromonas maricaloris sp. nov., isolated from an Australian sponge, and reclassification of [ Pseudoalteromonas aurantia ] NCIMB 2033 as Pseudoalteromonas flavipulchra sp. nov. Int J Syst Evol Microbiol 52:263–271
    [Google Scholar]
  16. Katayama-Fujimura Y., Komatsu Y., Kuraishi H., Kaneko T. 1984; Estimation of DNA base composition by high performance liquid chromatography of its nuclease P1 hydrolysate. Agric Biol Chem 48:3169–3172 [CrossRef]
    [Google Scholar]
  17. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  18. Kobayashi T., Kimura B., Fujii T. 2000; Haloanaerobium fermentans sp. nov., a strictly anaerobic, fermentative halophile isolated from fermented puffer fish ovaries. Int J Syst Evol Microbiol 50:1621–1627 [CrossRef]
    [Google Scholar]
  19. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184
    [Google Scholar]
  20. Macián M. C., Ludwig W., Schleifer K. H., Garay E., Pujalte M. J. 2001; Thalassomonas viridans gen. nov. sp. nov., a novel marine γ -proteobacterium. Int J Syst Evol Microbiol 51:1283–1289
    [Google Scholar]
  21. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  22. Miyamoto K., Tsujibo H., Hikita Y. 7 other authors 1998; Cloning and nucleotide sequence of the gene encoding a serine proteinase inhibitor named marinostatin from a marine bacterium Alteromonas sp. strain B-10-31. Biosci Biotechnol Biochem 62:2446–2449 [CrossRef]
    [Google Scholar]
  23. Nakamura K., Hiraishi A., Yoshimi Y., Kawaharasaki M., Masuda K., Komagata Y. 1995; Microlunatus phosphovorus gen. nov., sp. nov. a new Gram-positive polyphosphate-accumulating bacterium isolated from activated sludge. Int J Syst Bacteriol 45:17–22 [CrossRef]
    [Google Scholar]
  24. Romanenko L. A., Zhukova N. V., Lysenko A. M., Mikhailov V. V., Stackebrandt E. 2003a; Assignment of ‘ Alteromonas marinoglutinosa ’ NCIMB 1770 to Pseudoalteromonas mariniglutinosa sp. nov., nom. rev., comb. nov. Int J Syst Evol Microbiol 531105–1109 [CrossRef]
    [Google Scholar]
  25. Romanenko L. A., Zhukova N. V., Rohde M., Lysenko A. M., Mikhailov V. V., Stackebrandt E. 2003b; Pseudoalteromonas agarivorans sp. nov., a novel marine agarolytic bacterium. Int J Syst Evol Microbiol 53:125–131 [CrossRef]
    [Google Scholar]
  26. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  27. Sawabe T., Makino H., Tatsumi M., Nakano K., Tajima K., Iqbal M. M., Yumoto I., Ezura Y., Christen R. 1998; Pseudoalteromonas bacteriolytica sp. nov., a marine bacterium that is the causative agent of red spot disease of Laminaria japonica . Int J Syst Bacteriol 48:769–774 [CrossRef]
    [Google Scholar]
  28. Takano R., Imada C., Kamei K., Hara S. 1991; The reactive site of marinostatin, a proteinase inhibitor from marine Alteromonas sp. B-10-31. J Biochem 110:856–858
    [Google Scholar]
  29. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  30. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
    [Google Scholar]
  31. Yumoto I., Kawasaki K., Iwata H., Matsuyama H., Okuyama H. 1998; Assignment of Vibrio sp. strain ABE-1 to Colwellia maris sp. nov., a new psychrophilic bacterium. Int J Syst Bacteriol 48:1357–1362 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02516-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02516-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error