1887

Abstract

A marine bacterium producing protease inhibitors was isolated from neritic sea water and was studied phenotypically, genotypically and phylogenetically. This bacterium (strain B-10-31) produced three types of protease inhibitor, namely, marinostatin, monastatin and leupeptin, which were considerably different in terms of their chemical structure and properties. Strain B-10-31 was a rod-shaped, non-spore-forming, Gram-negative, strictly aerobic bacterium that was motile by means of one polar flagellum. The strain required Na for growth and exhibited optimal growth at 27 °C, pH 8·0 and 2 % (w/v) NaCl. It utilized various substrates, such as -glucose, maltose, maltotriose, -acetylglucosamine, -threonine, -serine, -arginine, -proline, --alanine and -glutamate, as the sole energy source. Ubiquinone-8 was the major respiratory quinone. The major fatty acids were C, C 7, C 9 and C 7. The G+C content of the DNA of strain B-10-31 was 42·0 mol%. Phylogenetic analysis, based on 16S rDNA sequences, showed that the strain clustered in the -. The aerobic marine bacterium was the species most closely related to the new isolate (90·4 % 16S rDNA sequence similarity); other described species in the - cluster showed low levels of sequence similarity with strain B-10-31 (<90 %). Based on the above results, it is proposed that the novel marine bacterium should be classified as a new species, for which the name (type strain B-10-31=JCM 11461=DSM 14643) is proposed.

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2003-11-01
2019-10-16
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vol. , part 6, pp. 1807-1811

A phylogenetic tree showing the position of B-10-31 among related taxa but constructed using a larger dataset than that used for Fig. 1 is available. [PDF]



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