1887

Abstract

A keratinolytic, nocardioform actinomycete, strain D2, was isolated from Kuwait marsh soil by the bait technique using animal wool. Strain D2 was an aerobic, Gram-positive organism that produced light-grey aerial mycelium but no specific spore chains. 16S rDNA sequence analyses and chemotaxonomic markers were consistent with the classification of strain D2 in the genus , i.e. it had -diaminopimelic acid in its peptidoglycan, arabinose and galactose as its diagnostic sugars, the polar lipids phosphatidylinositol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine and diphosphatidylglycerol, menaquinone MK-9(H) and an iso-/anteiso-branched fatty acid pattern combined with 10-methyl-branched and 2-hydroxy-branched fatty acids. was the closest phylogenetic neighbour of strain D2, showing 99·4 % 16S rDNA sequence similarity with the novel strain. and strain D2 could be clearly separated from each other on the basis of their low DNA–DNA reassociation value (55·9 %). These data, together with its distinct physiological traits, led to the conclusion that strain D2 represented a novel species within the genus , for which the name (type strain D2=DSM 44409=NRRL B24117) is proposed.

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2003-05-01
2019-10-19
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