1887

Abstract

Three strains of lactic acid bacteria, designated NJ 317, NJ 414 and NJ 415, were isolated from the outer leaves of Chinese cabbages ( L. var. Regel) and characterized taxonomically. The strains were Gram-reaction-positive, catalase-negative, facultatively anaerobic cocci that did not produce gas from glucose and formed -lactic acid. The major fatty acids were Cω9, C, C and summed feature 10. Morphological, physiological and phylogenetic data indicated that the strains belonged to the genus . These strains shared similar phenotypic characteristics and exhibited DNA relatedness values >96.6 % to each other, indicating that they represent a single species. The DNA G+C contents of the three strains were 42.1–42.5 mol%. 16S rRNA gene sequences of the novel strains were determined and aligned with those of other species of the genus . On the basis of phylogenetic analysis the three strains grouped with other members of the genus . and were the most closely related species, sharing a sequence similarity value of 94.4 % with the three strains. Ribotyping patterns, however, revealed that these strains were well-separated from reference strains of species of the genus and DNA–DNA hybridization studies indicated that the novel strains had low levels (<20.2 %) of DNA relatedness with reference strains of , and other type strains of previously described species, showing that they represent a different species. Based on this evidence, strains NJ 317, NJ 414 and NJ 415 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NJ 317 ( = JCM16395  = CGMCC 1.10453).

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2011-07-01
2019-10-19
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Micrograph of positively Gram-staied cells of strain NJ 317 . Bar, 50 μm.

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Colonies of strain NJ 317 incubated on MRS agar under anaerobic condition for 48 h. Bar, 10 mm.

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Cellular fatty acid composition of strain NJ 317 and type trains of species fo the genus . [PDF]

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