sp. nov., a marine bacterium of the family isolated from European sea bass Free

Abstract

A novel Gram-stain-negative rod-shaped gliding bacterial strain, designated 35/09, was isolated from diseased European sea bass ( L.) in Spain. Colonies were pale-yellow-pigmented with uneven edges and did not adhere to the agar. The DNA G+C content of the isolate was 31.3 mol%. 16S rRNA gene sequence analysis indicated affiliation to the genus (family , phylum ‘’). Sequence similarities between the isolate and type strains of other members of the genus were 93.1–97.3 %. The major fatty acids (>5 % of the total fatty acids) were iso-C (24.8 %), iso-C 3-OH (18.0 %), anteiso-C (8.1 %), Cω6 (6.9 %) and iso-C (6.2 %). Genotypic and phenotypic data indicate that strain 35/09 should be classified as a representative of a novel species in the genus , for which the name sp. nov. is proposed; the type strain is 35/09 ( = CECT 7612 = NCIMB 14598).

Funding
This study was supported by the:
  • Xunta de Galicia, Spain (Award INCITE 09E2R208063 ES, PGIDIT 08MMA017CT and  PGIDIT 07MMA012CT)
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2012-02-01
2024-03-29
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References

  1. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. 1996; Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46:128–148 [View Article]
    [Google Scholar]
  2. Bernardet J.-F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070 [View Article][PubMed]
    [Google Scholar]
  3. Buck J. D. 1982; Nonstaining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993[PubMed]
    [Google Scholar]
  4. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [View Article][PubMed]
    [Google Scholar]
  5. Choi D. H., Kim Y.-G., Hwang C. Y., Yi H., Chun J., Cho B. C. 2006; Tenacibaculum litoreum sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 56:635–640 [View Article][PubMed]
    [Google Scholar]
  6. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
    [Google Scholar]
  7. CLSI 2006; Methods for antimicrobial disk susceptibility testing of bacteria isolated from aquatic animals; approved guideline. CLSI Document M42-A, vol. 26, no. 23 Wayne, PA: Clinical and Laboratory Standards Institute;
  8. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  9. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
    [Google Scholar]
  10. Felsenstein F. 2007 phylip (phylogeny inference package) version 3.67. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  11. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  12. Frette L., Jørgensen N. O. G., Irming H., Kroer N. 2004; Tenacibaculum skagerrakense sp. nov., a marine bacterium isolated from the pelagic zone in Skagerrak, Denmark. Int J Syst Evol Microbiol 54:519–524 [View Article][PubMed]
    [Google Scholar]
  13. Hansen G. H., Bergh O., Michaelsen J., Knappskog D. 1992; Flexibacter ovolyticus sp. nov., a pathogen of eggs and larvae of Atlantic halibut, Hippoglossus hippoglossus L.. Int J Syst Bacteriol 42:451–458 [View Article][PubMed]
    [Google Scholar]
  14. Heindl H., Wiese J., Imhoff J. F. 2008; Tenacibaculum adriaticum sp. nov., from a bryozoan in the Adriatic Sea. Int J Syst Evol Microbiol 58:542–547 [View Article][PubMed]
    [Google Scholar]
  15. Huß V. A. R., Festl H., Scheifer K. H. 1983; Studies on the spectophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  16. Jung S.-Y., Oh T.-K., Yoon J.-H. 2006; Tenacibaculum aestuarii sp. nov., isolated from a tidal flat sediment in Korea. Int J Syst Evol Microbiol 56:1577–1581 [View Article][PubMed]
    [Google Scholar]
  17. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
    [Google Scholar]
  18. Lee Y. S., Baik K. S., Park S. Y., Kim E. M., Lee D. H., Kahng H. Y., Jeon C. O., Jung J. S. 2009; Tenacibaculum crassostreae sp. nov., isolated from the Pacific oyster, Crassostrea gigas . Int J Syst Evol Microbiol 59:1609–1614 [View Article][PubMed]
    [Google Scholar]
  19. MacFaddin J. F. 1976 Biochemical Tests for Identification of Medical Bacteria Baltimore, MD: Williams and Wilkins;
    [Google Scholar]
  20. Pazos F., Santos Y., Macías A. R., Núñez S., Toranzo A. E. 1996; Evaluation of media for the successful culture of Flexibacter maritimus . J Fish Dis 19:193–197 [View Article]
    [Google Scholar]
  21. Piñeiro-Vidal M., Riaza A., Santos Y. 2008a; Tenacibaculum discolor sp. nov. and Tenacibaculum gallaicum sp. nov., isolated from sole (Solea senegalensis) and turbot (Psetta maxima) culture systems. Int J Syst Evol Microbiol 58:21–25 [View Article][PubMed]
    [Google Scholar]
  22. Piñeiro-Vidal M., Carballas C. G., Gómez-Barreiro O., Riaza A., Santos Y. 2008b; Tenacibaculum soleae sp. nov., isolated from diseased sole (Solea senegalensis Kaup). Int J Syst Evol Microbiol 58:881–885 [View Article][PubMed]
    [Google Scholar]
  23. Reichenbach H. 1989; Order I. Cytophagales Leadbetter 1974. In Bergey’s Manual of Systematic Bacteriology vol. 3 pp. 2011–2013 Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  24. Reichenbach H. 1992a; The order Cytophagales . In The Prokaryotes, 2nd edn. vol. 4 pp. 3631–3675 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer; [CrossRef]
    [Google Scholar]
  25. Reichenbach H. 1992b; Flavobacteriaceae fam. nov. In Validation of the Publication of the New Names and New Combinations Previously Effectively Published Outside the IJSB, List no. 41. Int J Syst Bacteriol 42:327–329 [View Article]
    [Google Scholar]
  26. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  27. Sasser M. 1990; Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc;
  28. Sheu S.-Y., Lin K.-Y., Chou J.-H., Chang P.-S., Arun A. B., Young C.-C., Chen W.-M. 2007; Tenacibaculum litopenaei sp. nov., isolated from a shrimp mariculture pond. Int J Syst Evol Microbiol 57:1148–1153 [View Article][PubMed]
    [Google Scholar]
  29. Suzuki M. 2011; Genus LIII. Tenacibaculum Suzuki, Nakagawa, Harayama and Yamamoto 2001, 1650VP . In Bergey’s Manual of Systematic Bacteriology, 2nd edn. vol. 4 pp. 279–282 Edited by Krieg N. R., Ludwig W., Whitman W. B., Hedlund B. P., Paster B. J., Staley J. T., Ward N., Brown D., Parte A. New York: Springer;
    [Google Scholar]
  30. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. 2001; Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov.. Int J Syst Evol Microbiol 51:1639–1652 [View Article][PubMed]
    [Google Scholar]
  31. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
    [Google Scholar]
  32. Wakabayashi H., Hikida M., Masumura K. 1986; Flexibacter maritimus sp. nov., a pathogen of marine fishes. Int J Syst Bacteriol 36:396–398 [View Article]
    [Google Scholar]
  33. Wang J. T., Chou Y. J., Chou J. H., Chen C. A., Chen W. M. 2008; Tenacibaculum aiptasiae sp. nov., isolated from a sea anemone Aiptasia pulchella . Int J Syst Evol Microbiol 58:761–766 [View Article][PubMed]
    [Google Scholar]
  34. Yoon J.-H., Kang S.-J., Oh T.-K. 2005; Tenacibaculum lutimaris sp. nov., isolated from a tidal flat in the Yellow Sea, Korea. Int J Syst Evol Microbiol 55:793–798 [View Article][PubMed]
    [Google Scholar]
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