1887

Abstract

An actinomycete strain, designated IR27-S3, was isolated from a forest soil sample collected from Iriomote Island, Okinawa, Japan. Cells of the isolate were Gram-stain-positive, aerobic, non-sporulating, non-motile coccoids or short rods. The strain grew in the presence of 0–7 % (w/v) NaCl, at pH 6–8 and at 12–37 °C, with optimum growth at 30 °C. Chemotaxonomically, the strain contained -diaminopimelic acid as the diagnostic diamino acid and MK-8(H) as the predominant menaquinone. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown phospholipid. The major cellular fatty acids were iso-C, C -9, C and iso-C. The DNA G+C content was 73.7 mol%. On the basis of 16S rRNA gene sequence analysis, strain IR27-S3 was closely related to MSL-22 (98.1 % 16S rRNA gene sequence similarity), MSL-05 (97.2 %) and DSM 20641 (96.5 %). On the basis of fatty acid analysis, phylogenetic analysis and phenotypic data, the isolate should be classified in a novel species of the genus , for which the name sp. nov. is proposed. The type strain is IR27-S3 ( = NBRC 105384  = KACC 14926).

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2011-09-01
2019-10-18
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vol. , part 9, pp. 2205 - 2209

Scanning electron micrograph of cells of sp. nov. IR27-S3 grown on tryptic soy agar at 30 °C for 14 days. Bar, 1 µm.



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