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Abstract

Strain YSM-79 was isolated from commercial salt made from seawater in Yonaguni island, Okinawa, Japan. The strain is an aerobic, Gram-negative, chemo-organotrophic and extremely halophilic archaeon. Cells are short rods that lyse in distilled water. Growth occurs at 1.5–5.3 M NaCl (optimum 2.5–3.0 M), pH 5.0–8.8 (optimum pH 5.2–6.3) and 20–55 °C (optimum 40 °C). Mg is required for growth, with maximum growth at 200–300 mM Mg. Polar lipid analysis revealed the presence of phosphatidylglycerol, phosphatidylglycerophosphate methyl ester, sulfated diglycosyl diether-1 and five unidentified glycolipids. The G+C content of the DNA was 64 mol%. On the basis of 16S rRNA gene sequence analysis, strain YSM-79 was determined to be a member of the family , with the closest related genus being (94 % sequence identity). In addition, the ′ gene sequence of strain YSM-79 had <88 % sequence similarity to those of other members of the family . The results of phenotypic, chemotaxonomic and phylogenetic analysis suggested that strain YSM-79 should be placed in a new genus, gen. nov., as a representative of sp. nov. The type strain is YSM-79 ( = JCM 16327  = CECT 7563).

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2011-09-01
2020-01-21
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Supplements

vol. , part 9, pp. 2266–2270

Phase-contrast micrograph of strain YSM-79

TLC of polar lipids of strain YSM-79 and other reference haloarchaea

Maximum-likelihood phylogenetic tree based on ′ gene sequences, showing the positions of strain YSM-79 and some other related haloarchaeal strains

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