1887

Abstract

A polyphasic taxonomic study was performed on a novel facultatively anaerobic, hydrogen- or sulfur/thiosulfate-oxidizing, thermophilic chemolithoautotroph recently isolated from subsurface hot aquifer water in a Japanese gold mine. The cells were straight to slightly curved rods, with a single polar flagellum. Growth was observed at 40–70 °C (optimum 60–65 °C; 80 min doubling time) and at pH 6·4–8·8 (optimum pH 7·5). The isolate was unable to use complex organic compounds, carbohydrates, amino acids or organic acids as sole energy and carbon sources. The G+C content of the genomic DNA was 31·3 mol%. Phylogenetic analysis based on 16S rDNA sequences indicated that the isolate was closely related to an uncultivated group of micro-organisms within the order obtained from Icelandic and Japanese hot spring microbial mats, but distantly related to previously identified genera of the such as , and . The name gen. nov., sp. nov. is proposed for this novel species; the type strain is HGMK1 (=JCM 11477=ATCC BAA-562=DSM 15120).

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2003-05-01
2020-01-22
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vol. , part 3, pp. 823 - 827

Effects of temperature (A), pH (B) and sea salts concentration (C) on the growth of gen. nov., sp. nov. Growth curves at different temperatures were determined in mjANHOX medium at pH 7.5. The effect of pH on growth was determined in mjANHOX medium containing various buffers at 65 °C over a range of pH values. The pH was adjusted at room temperature. The effect of sea salts concentration on growth was determined at 65 °C and pH 7.5. [PDF](14 KB)



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