RT Journal Article SR Electronic(1) A1 Li, Qin Qin A1 Wang, En Tao A1 Chang, Yue Li A1 Zhang, Yun Zeng A1 Zhang, Yan Ming A1 Sui, Xin Hua A1 Chen, Wen Feng A1 Chen, Wen XinYR 2011 T1 Ensifer sojae sp. nov., isolated from root nodules of Glycine max grown in saline-alkaline soils JF International Journal of Systematic and Evolutionary Microbiology, VO 61 IS 8 SP 1981 OP 1988 DO https://doi.org/10.1099/ijs.0.025049-0 PB Microbiology Society, SN 1466-5034, AB Thirteen bacterial isolates from root nodules of soybean grown in saline-alkaline soils in the Chinese province of Hebei were identified as a unique group in the genus Ensifer based upon BOX-PCR patterns, sequencing analyses of 16S rRNA and housekeeping genes and DNA–DNA hybridization. Phenotypically, positive tests for acid production and negative results for reduction in litmus milk and sensitivity to 50 µg ampicillin ml−1, as well as some other features, could differentiate the novel group from defined species of the Ensifer–Sinorhizobium group. The novel group had symbiotic gene sequences (nodC and nifH) that were identical or very similar to those of Ensifer (Sinorhizobium) fredii, and formed effective nodules with Glycine max (soybean), Vigna unguiculata and Glycine soja. Based upon the consensus of these analyses, a novel species, Ensifer sojae sp. nov., is proposed, with CCBAU 05684T ( = LMG 25493T  = HAMBI 3098T) as the type strain. The DNA G+C content of strain CCBAU 05684T was 60.9 mol% (T m)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.025049-0