1887

Abstract

A Gram-stain-negative, non-motile, non-spore-forming bacterial strain, BDR-9, was isolated from soil collected from Boryung on the west coast of the Korean peninsula, and its taxonomic position was investigated by using a polyphasic study. Strain BDR-9 grew optimally at 25 °C, at pH 6.0–7.5 and in the absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain BDR-9 fell within the clade comprising species of the genus within the phylum . 16S rRNA gene sequence similarity values between strain BDR-9 and the type strains of species of the genus were in the range 94.0–95.6 %. Strain BDR-9 contained MK-7 as the predominant menaquinone and iso-C and Cω7 and/or iso-C 2-OH as the major fatty acids. The DNA G+C content was 44.3 mol%. Differential phenotypic properties and phylogenetic distinctiveness of strain BDR-9 demonstrated that this strain is distinguishable from species of the genus . On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain BDR-9 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BDR-9 ( = KCTC 23157  = CCUG 59599).

Funding
This study was supported by the:
  • , Program for Collection, Management and Utilization of Biological Resources , (Award M10867010003)
  • , 21C Frontier Program of Microbial Genomics and Applications , (Award MG05-0401-2-0)
  • , Ministry of Education, Science and Technology (MEST) of the Republic of Korea
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2011-07-01
2021-03-04
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References

  1. An D.-S., Yin C.-R., Lee S.-T., Cho C.-H. 2009; Mucilaginibacter daejeonensis sp. nov., isolated from dried rice straw. Int J Syst Evol Microbiol 59:1122–1125 [CrossRef][PubMed]
    [Google Scholar]
  2. Baik K. S., Park S. C., Kim E. M., Lim C. H., Seong C. N. 2010; Mucilaginibacter rigui sp. nov., isolated from wetland freshwater, and emended description of the genus Mucilaginibacter . Int J Syst Evol Microbiol 60:134–139 [CrossRef][PubMed]
    [Google Scholar]
  3. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:1861–1868[PubMed]
    [Google Scholar]
  4. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006[PubMed] [CrossRef]
    [Google Scholar]
  5. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  6. Jeon Y., Lee S.-S., Chung B. S., Kim J. M., Bae J.-W., Park S. K., Jeon C. O. 2009; Mucilaginibacter oryzae sp. nov., isolated from soil of a rice paddy. Int J Syst Evol Microbiol 59:1451–1454 [CrossRef][PubMed]
    [Google Scholar]
  7. Komagata K., Suzuki K. 1987; Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [CrossRef]
    [Google Scholar]
  8. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [CrossRef]
    [Google Scholar]
  9. Luo X., Zhang L., Dai J., Liu M., Zhang K., An H., Fang C. 2009; Mucilaginibacter ximonensis sp. nov., isolated from Tibetan soil. Int J Syst Evol Microbiol 59:1447–1450 [CrossRef][PubMed]
    [Google Scholar]
  10. Pankratov T. A., Tindall B. J., Liesack W., Dedysh S. N. 2007; Mucilaginibacter paludis gen. nov., sp. nov. and Mucilaginibacter gracilis sp. nov., pectin-, xylan- and laminarin-degrading members of the family Sphingobacteriaceae from acidic Sphagnum peat bog. Int J Syst Evol Microbiol 57:2349–2354 [CrossRef][PubMed]
    [Google Scholar]
  11. Reichenbach H. 1992; The order Cytophagales . In The Prokaryotes, 2nd edn. vol. 4 pp. 3631–3675 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer; [CrossRef]
    [Google Scholar]
  12. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids Technical Note 101 Newark, DE: MIDI, Inc;
    [Google Scholar]
  13. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  14. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  15. Urai M., Aizawa T., Nakagawa Y., Nakajima M., Sunairi M. 2008; Mucilaginibacter kameinonensis sp. nov., isolated from garden soil. Int J Syst Evol Microbiol 58:2046–2050 [CrossRef][PubMed]
    [Google Scholar]
  16. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [CrossRef]
    [Google Scholar]
  17. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [CrossRef][PubMed]
    [Google Scholar]
  18. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H. 2003; Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53:53–57 [CrossRef][PubMed]
    [Google Scholar]
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