1887

Abstract

Two strains, CA15-8 and CA15-9, were isolated from soil, under alkali conditions, and characterized phenotypically, chemotaxonomically and genetically. These alkalitolerant organisms grew over a wide pH range (pH 7·0–12·0), with an optimum at pH 7·0–8·0. The mean G+C content of the DNA of these strains was 63±2 mol%. The strains contained MK-8(H) and MK-9(H) as the main respiratory quinones. The cell-wall peptidoglycan was Lys-Thr-Ala and the whole-cell sugar was rhamnose. The major cellular fatty acids of the isolates were anteiso-C and iso-C. A phylogenetic analysis, based on 16S rDNA sequence data, revealed that strains CA15-8 and CA15-9 formed an evolutionary lineage distinct from other species. On the basis of morphological, physiological and chemotaxonomic characteristics, 16S rDNA sequence comparisons and DNA–DNA hybridization data, a novel species of is proposed, namely (type strain CA15-8=KCTC 9922=IFO 16787).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02492-0
2003-09-01
2020-01-17
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/53/5/ijs531277.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02492-0&mimeType=html&fmt=ahah

References

  1. Conn, J. & Dimmick, I. ( 1947; ). Soil bacteria similar in morphology to Mycobacterium and Corynebacterium. J Bacteriol 54, 291–303.
    [Google Scholar]
  2. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  3. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5c. Department of Genetics, University of Washington, Seattle, USA.
  4. Funke, G., von Graevenitz, A., Clarridge, J. E., III & Bernard, K. A. ( 1997; ). Clinical microbiology of coryneform bacteria. Clin Microbiol Rev 10, 125–159.
    [Google Scholar]
  5. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  6. Koch, C., Schumann, P. & Stackebrandt, E. ( 1995; ). Reclassification of Micrococcus agilis (Ali-Cohen 1889) to the genus Arthrobacter as Arthrobacter agilis comb. nov. and emendation of the genus Arthrobacter. Int J Syst Bacteriol 45, 837–839.[CrossRef]
    [Google Scholar]
  7. Kodama, Y., Yamamoto, H., Amano, N. & Amachi, T. ( 1992; ). Reclassification of two strains of Arthrobacter oxydans and proposal of Arthrobacter nicotinovorans sp. nov. Int J Syst Bacteriol 42, 234–239.[CrossRef]
    [Google Scholar]
  8. Komagata, K. & Suzuki, K.-I. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. In Methods in Microbiology, vol. 19, pp. 161–207. Edited by R. R. Colwell & R. Grigorova. New York: Academic Press.
  9. Lee, J.-S., Jung, M.-C., Kim, W.-S. & 10 other authors ( 1996; ). Identification of lactic acid bacteria from kimchi by cellular FAMEs analysis. Kor J Appl Microbiol Biotechnol 24, 234–241.
    [Google Scholar]
  10. Marmur, J. ( 1961; ). A procedure for the isolation of DNA from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  11. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  12. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  13. Shin, Y. K., Lee, J.-S., Chun, C. O., Kim, H.-J. & Park, Y.-H. ( 1996; ). Isoprenoid quinone profiles of the Leclercia adecarboxylata KCTC 1036T. J Microbiol Biotechnol 6, 68–69.
    [Google Scholar]
  14. Skerman, V. B. D. ( 1967; ). A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins.
  15. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  16. Stackebrandt, E. & Liesack, W. ( 1993; ). Nucleic acids and classification. In Handbook of New Bacterial Systematics, pp. 152–189. Edited by M. Goodfellow & A. G. O'Donnell. London: Academic Press.
  17. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  18. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  19. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  20. Yang, P., Vauterin, L., Vancanneyt, M., Swings, J. & Kersters, K. ( 1993; ). Application of fatty acid methyl esters for the taxonomic analysis of the genus Xanthomonas. Syst Appl Microbiol 16, 47–71.
    [Google Scholar]
  21. Yumoto, I., Yamazaki, K., Sawabe, T., Nakano, K., Kawasaki, K., Ezura, Y. & Shinano, H. ( 1998; ). Bacillus horti sp. nov., a new gram-negative alkaliphilic bacillus. Int J Syst Bacteriol 48, 565–571.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02492-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02492-0
Loading

Data & Media loading...

Supplements

vol. , part 5, pp. 1277-1280

A tree, based on 16S rDNA sequences, showing the phylogenetic position of with respect to members of the genus can be obtained when you click here(PDF file).



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error