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Abstract

During the taxonomic investigation of cold-adapted bacteria from samples collected in the Antarctic area of the South Shetland Islands, one Gram-reaction-negative, psychrotolerant, aerobic bacterium, designated strain M1, was isolated from marine sediment collected on Deception Island. The organism was rod-shaped, catalase- and oxidase-positive and motile by means of a polar flagellum. This psychrotolerant strain grew at temperatures ranging from −4 °C to 34 °C. Phylogenetic studies based on 16S rRNA gene sequences confirmed that Antarctic isolate M1 was a member of the genus and was located in the cluster. 16S rRNA gene sequence similarity values were >98 % between 13 type strains belonging to the lineage. However, phylogenetic analysis of gene sequences showed that strain M1 exhibited high sequence similarity only with respect to (97.42 %) and (96.40 %) and DNA–DNA hybridization experiments between the Antarctic isolate M1 and the type strains of these two closely related species revealed relatedness values of 58 and 57 %, respectively. Several phenotypic characteristics, together with the results of polar lipid and cellular fatty acid analyses, were used to differentiate strain M1 from related pseudomonads. Based on the evidence of this polyphasic taxonomic study, strain M1 represents a novel species, for which the name sp. nov. is proposed. The type strain is M1 ( = LMG 25555  = CECT 7677).

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2011-10-01
2020-01-21
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Electron micrograph of a negatively stained cell of strain M1 showing a single polar flagellum. Bar, 1 μm.

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Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences of strain M1 , the type strain of and closely related type strains of the genus . [PDF](37KB)

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Neighbour-joining phylogenetic tree based on gene sequences of strain M1 and closely related type strains of the genus . [PDF](35KB)

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Two-dimensional TLC of polar lipids of strain M1 . AL1-3, unknown aminolipids; DPG, diphosphatidylglycerol; GPL, glucophospholipid; L1-13, unknown polar lipids; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PL, phospholipid.

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Cellular fatty acid composition of strain M1 and its closest phylogenetic neighbours. Highest 16S rRNA and gene sequence similarities between strain M1 and the type strains of species fo the genus . Supplementary Tables [PDF](35KB)

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