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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 53, Issue 5

sp. nov. and sp. nov., dimethylsulfoniopropionate-demethylating bacteria from marine environments Free

Abstract

Three Gram-negative, rod-shaped, aerobic bacteria that were capable of degrading dimethylsulfoniopropionate (DMSP) were isolated from marine waters. These isolates (DSS-3, DSS-10 and ISM) exhibited the ability to demethylate and cleave DMSP, as well as to degrade other sulfur compounds related to DMSP that are cycled in marine environments. Intracellular poly--hydroxybutyrate inclusions, surface blebs and one polar, complex flagellum that rotated exclusively in the clockwise direction were observed for DSS-3. The outer membrane of ISM was separated from the cytoplasm at the poles in a toga-like morphology. The primary fatty acid in both strains was C 7. DNA G+C contents for the isolates were 68·0±0·1, 68·1±0·1 and 66·0±0·2 mol% for DSS-3, DSS-10 and ISM, respectively. 16S rRNA gene sequence analyses placed these organisms within the lineage of the -. Closely related species were and (DSS-3 and DSS-10) and (ISM). Neither DSS-3 nor ISM exhibited 16S rRNA similarity >97 % or DNA–DNA hybridization values >45 % to their nearest described relatives. Genotypic and phenotypic analyses support the creation of two novel species: sp. nov. with strain DSS-3 (=ATCC 700808=DSM 15171) as the type strain, and sp. nov. with strain ISM (=ATCC BAA-591=DSM 15170) as the type strain.

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Keyword(s): DMS, dimethylsulfide , DMSP, dimethylsulfoniopropionate; , ITS, internal transcribed spacer , MeSH, methanethiol and PHB, poly-β-hydroxybutyrate
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2003-09-01
2024-04-16
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Silicibacter pomeroyi sp. nov. and Roseovarius nubinhibens sp. nov., dimethylsulfoniopropionate-demethylating bacteria from marine environments
Int J Syst Evol Microbiol 53, 1261 (2003); https://doi.org/10.1099/ijs.0.02491-0
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