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Abstract

Two thermophilic, Gram-stain-positive, sporulating bacterial strains, which formed branched vegetative and aerial mycelia, were isolated from fallen leaves sampled from geothermal soils and designated ONI-1 and ONI-5. Strain ONI-1 grew at 50–74 °C, with optimum growth at 60–65 °C, and strain ONI-5 grew at 45–74 °C, with optimum growth at 60–65 °C. The pH range for growth of the strains was pH 4.6–8.0, with optimum growth at pH 7.0. The DNA G+C contents of strains ONI-1 and ONI-5 were 60.2 and 58.1 mol%, respectively. The major fatty acid was iso-C and the major menaquinone was MK-9(H). The cell walls of the strains contained glutamic acid, serine, glycine, histidine, alanine and ornithine. The polar lipids consisted of phosphatidylinositol, phosphatidylglycerol and a glycolipid. The cell-wall sugar was rhamnose. Detailed phylogenetic analysis based on 16S rRNA gene sequences indicated that the strains belong to the class and that strains ONI-1 and ONI-5 are most closely related to SK20-1 (85.3 and 84.5 % sequence similarity, respectively). 16S rRNA gene sequence similarity between the two strains was 96.6 %. Based on the phenotypic features and phylogenetic position, we propose that strains ONI-1 and ONI-5 constitute a novel genus containing two novel species, for which we propose the names gen. nov., sp. nov. (the type species; type strain ONI-1  = JCM 16817  = KCTC 19768) and sp. nov. (type strain ONI-5  = JCM 16818  = KCTC 19767), within the new family fam. nov. and order ord. nov.

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2011-04-01
2020-01-23
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