1887

Abstract

An unknown Gram-positive, catalase-negative, facultatively anaerobic, non-spore-forming, rod-shaped bacterium originating from semen of a pig was characterized using phenotypic, molecular chemical and molecular phylogenetic methods. Chemical studies revealed the presence of a directly cross-linked cell wall murein based on -lysine and a DNA G+C content of 39 mol%. Comparative 16S rRNA gene sequencing showed that the unidentified rod-shaped organism formed a hitherto unknown subline related, albeit loosely, to , , and related organisms, in the low-G+C-content Gram-positive bacteria. However, sequence divergence values of >11 % from these recognized taxa clearly indicated that the novel bacterium represents a separate genus. Based on phenotypic and phylogenetic considerations, it is proposed that the unknown bacterium from pig semen be classified as a new genus and species, gen. nov., sp. nov. The type strain is strain 01-570-1 (=CCUG 45438=CIP 107425).

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2003-05-01
2020-08-10
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