Allofustis seminis gen. nov., sp. nov., a novel Gram-positive, catalase-negative, rod-shaped bacterium from pig semen

Matthew D. Collins; Robert Higgins; Serge Messier; Madeleine Fortin; Roger A. Hutson; Paul A. Lawson; Enevold Falsen

doi:10.1099/ijs.0.02484-0

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Allofustis seminis gen. nov., sp. nov., a novel Gram-positive, catalase-negative, rod-shaped bacterium from pig semen

Matthew D. Collins¹, Robert Higgins², Serge Messier², Madeleine Fortin², Roger A. Hutson¹, Paul A. Lawson¹ and Enevold Falsen³
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Affiliations: ¹ School of Food Biosciences, University of Reading, Whiteknights, Reading RG6 6AP, UK ² Université de Montréal, Faculté de Médecine Vétérinaire, St Hyacinthe, Quebec, Canada ³ Culture Collection, Department of Clinical Bacteriology, University of Göteborg, Sweden
CorrespondenceMatthew D. Collins  [email protected]
Published: 01 May 2003 https://doi.org/10.1099/ijs.0.02484-0

Abstract

An unknown Gram-positive, catalase-negative, facultatively anaerobic, non-spore-forming, rod-shaped bacterium originating from semen of a pig was characterized using phenotypic, molecular chemical and molecular phylogenetic methods. Chemical studies revealed the presence of a directly cross-linked cell wall murein based on l-lysine and a DNA G+C content of 39 mol%. Comparative 16S rRNA gene sequencing showed that the unidentified rod-shaped organism formed a hitherto unknown subline related, albeit loosely, to Alkalibacterium olivapovliticus, Alloiococcus otitis, Dolosigranulum pigrum and related organisms, in the low-G+C-content Gram-positive bacteria. However, sequence divergence values of >11 % from these recognized taxa clearly indicated that the novel bacterium represents a separate genus. Based on phenotypic and phylogenetic considerations, it is proposed that the unknown bacterium from pig semen be classified as a new genus and species, Allofustis seminis gen. nov., sp. nov. The type strain is strain 01-570-1T (=CCUG 45438T=CIP 107425T).

Published Online: 01/05/2003

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Allofustis seminis gen. nov., sp. nov., a novel Gram-positive, catalase-negative, rod-shaped bacterium from pig semen

Int J Syst Evol Microbiol 53, 811 (2003); https://doi.org/10.1099/ijs.0.02484-0

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Volume 53, Issue 3

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Allofustis seminis gen. nov., sp. nov., a novel Gram-positive, catalase-negative, rod-shaped bacterium from pig semen

Abstract

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