This study was set up to identify three Gram-negative, rod-shaped strains originating from broiler meat packaged under a modified atmosphere. A polyphasic taxonomic approach, including multilocus sequence analysis (MLSA) of five genes (16S rRNA, , , and ), DNA–DNA reassociation between the closest phylogenetic neighbours and determination of relevant phenotypic properties, was applied. Phylogenetic analysis of the 16S rRNA gene sequences grouped these strains together and within the genus . MLSA of the 16S rRNA gene and four housekeeping genes showed that the strains formed a monophyletic group separate from other species in all phylogenetic trees constructed. The strains had a phenotypic profile different from those of other representatives of the genus , but most similar to that of . Typical virulence markers for pathogenic were not detected. Based on phylogenetic, phenotypic and DNA–DNA reassociation data, a novel species, sp. nov., is proposed for the isolated strains. The type strain is APN3a-c ( = DSM 22296  = LMG 25213).


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vol. , part 10, pp. 2368–2372

GenBank accession numbers of , , and gene sequences used in this study

RI and dIII ribopatterns and dendrograms

Maximum-parsimony tree based on 16S rRNA gene sequences of sp. nov. and type and reference strains

Maximum-parsimony tree based on concatenated , , and gene sequences of sp. nov. and type and reference strains

Neighbour-joining tree based on 16S rRNA gene sequences of members of the genus and type species of genera in the family

Transmission electron micrograph of negatively stained cells of sp. nov. APN 3a-c

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